The PRKACG antibody is designed to detect the cAMP-dependent protein kinase catalytic subunit gamma (PRKACG), a key enzyme in the cAMP signaling pathway. PRKACG, encoded by the *PRKACG* gene, is one of three catalytic subunits (alpha, beta, gamma) of protein kinase A (PKA), a serine/threonine kinase critical for regulating cellular responses to cAMP. Unlike the ubiquitously expressed PRKACA and PRKACB isoforms, PRKACG exhibits tissue-specific expression, primarily in testis and skeletal muscle, and plays specialized roles in sperm maturation, motility, and metabolic regulation.
Antibodies targeting PRKACG are essential tools for studying its expression, localization, and function in physiological and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate PKA signaling dynamics, particularly in reproductive biology, muscle function, and cAMP-related disorders. Dysregulation of PRKACG has been implicated in infertility, metabolic syndromes, and certain cancers, making its detection crucial for mechanistic and diagnostic research. However, cross-reactivity with other PKA catalytic subunits remains a challenge, necessitating rigorous validation using knockout controls or isoform-specific epitopes. Recent studies also explore PRKACG's role in non-canonical pathways, such as crosstalk with mTOR or Wnt signaling, expanding its relevance in cellular homeostasis and disease.