Ring1抗体

+ +

•  

   

     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

•  

   

     Western blot analysis using Ring1 mouse mAb against MOLT-4 (1), LNCaP (2), Hela (3), HEK-293 (4) and Jurkat (5) cell lysate.    

  
  

•  

   

     Flow cytometric analysis of Hela cells using Ring1 mouse mAb (green) and negative control (red).    

  
  

•  

   

     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using Ring1 mouse mAb with DAB staining.    

  
  

•  

   

     Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using Ring1 mouse mAb with DAB staining.    

  
  

Ring1 antibodies target proteins within the Polycomb Repressive Complex 1 (PRC1), a critical epigenetic regulator. The Ring1 family, primarily Ring1A (RING1) and Ring1B (RNF2), functions as E3 ubiquitin ligases that catalyze monoubiquitination of histone H2A at lysine 119 (H2AK119ub1), a hallmark of transcriptionally repressive chromatin. Ring1B is the predominant catalytic subunit in PRC1. essential for maintaining gene silencing during development, stem cell differentiation, and cellular identity. Dysregulation of Ring1 proteins is linked to cancers, developmental disorders, and metabolic diseases.

Antibodies against Ring1 are widely used to study PRC1 dynamics in chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting. They help elucidate PRC1's role in recruiting other repressive complexes (e.g., PRC2) and its tissue-specific interactions with paralogs like PCGF or PHC proteins. Specificity is critical due to structural homology between Ring1A and Ring1B; many antibodies are isoform-selective, validated via knockout cell lines. Commercial variants (e.g., Millipore’s D7B8R, Abcam’s EPR5747) are optimized for distinct applications, such as detecting H2AK119ub1-associated chromatin regions or quantifying PRC1 levels in cancer models. Research using Ring1 antibodies has revealed their oncogenic or tumor-suppressive roles depending on cellular context, highlighting their therapeutic targeting potential in diseases like leukemia or solid tumors.