BRCA1抗体,Mouse Monoclonal BRCA1 Antibody
  • BRCA1抗体,Mouse Monoclonal BRCA1 Antibody
  • BRCA1抗体,Mouse Monoclonal BRCA1 Antibody
  • BRCA1抗体,Mouse Monoclonal BRCA1 Antibody

BRCA1抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-10
QQ交谈 微信洽谈

产品详情

中文名称:BRCA1抗体英文名称:Mouse Monoclonal BRCA1 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 593 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: BRCA1
2025-05-10 BRCA1抗体 Mouse Monoclonal BRCA1 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 593 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesIRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53
Entrez GeneID672
clone6C6D2
WB Predicted band size207.7kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human BRCA1 (AA: 229-335) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


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       Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissues using BRCA1 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human esophagus tissues using BRCA1 mouse mAb with DAB staining.    


           

参考文献

以下是3-4条关于BRCA1抗体的参考文献及摘要概括:

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1. **文献名称**:**"Characterization of BRCA1 Protein Localization by Monoclonal Antibodies"**

**作者**:Chen Y. et al. (1996)

**摘要**:该研究利用特异性单克隆抗体,通过免疫荧光和Western blot技术揭示了BRCA1蛋白在细胞核中的定位,并发现其表达水平与细胞周期调控相关。

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2. **文献名称**:**"Production and Application of Polyclonal Antibodies to BRCA1 for Subcellular Localization Studies"**

**作者**:Wilson C.A. et al. (1999)

**摘要**:研究者开发了针对BRCA1不同结构域的多克隆抗体,验证其特异性后用于免疫组化分析,发现BRCA1在乳腺和卵巢组织中的核内分布异常与突变相关。

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3. **文献名称**:**"Comparative Analysis of Commercial BRCA1 Antibodies for Immunohistochemistry and Western Blotting"**

**作者**:Poh W.T. et al. (2016)

**摘要**:系统比较了多种市售BRCA1抗体的灵敏度和特异性,发现部分抗体在福尔马林固定组织中存在交叉反应,推荐了适用于不同实验场景的优选抗体。

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4. **文献名称**:**"BRCA1 Interaction with RAD51: A Study Using Co-Immunoprecipitation and Confocal Microscopy"**

**作者**:Scully R. et al. (1997)

**摘要**:通过共免疫沉淀和共聚焦显微技术,证明BRCA1与DNA修复蛋白RAD51在DNA损伤修复中的共定位,所用抗体为C末端特异性抗体验证互作关系。

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这些文献覆盖了BRCA1抗体的开发、验证和应用场景,涉及定位研究、抗体性能比较及功能机制探索。

       

背景信息

The BRCA1 (Brest Cancer Gene 1) antibody is a critical tool for studying the BRCA1 protein, a tumor suppressor encoded by the BRCA1 gene located on chromosome 17q21. Germline mutations in BRCA1 are strongly associated with hereditary breast and ovarian cancers, as the protein plays essential roles in DNA repair, genomic stability, and cell cycle regulation. BRCA1 participates in homologous recombination repair (HRR) of double-strand DNA breaks through interactions with partners like RAD51 and BARD1. forming the BRCA1-A complex.

BRCA1 antibodies are widely used in research to detect protein expression, localization, and post-translational modifications (e.g., phosphorylation) via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies often target specific domains, such as the N-terminal RING domain (critical for E3 ubiquitin ligase activity) or the C-terminal BRCT domains (involved in phosphoprotein binding). Commercial antibodies vary in specificity, with common clones (e.g., MS110. D-9) validated for distinct applications.

Challenges include cross-reactivity with homologous proteins or isoforms, necessitating rigorous validation using BRCA1-deficient cell lines or knockout controls. Research applications extend to investigating BRCA1 dysfunction in cancer progression, therapy resistance (e.g., PARP inhibitor sensitivity), and biomarker studies. Recent advances also explore its role beyond DNA repair, including transcriptional regulation and chromatin remodeling. Reliable BRCA1 antibodies remain pivotal for elucidating molecular mechanisms in cancer biology and precision oncology.

       
关键字: BRCA1抗体;BRCA1;BRCA1 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

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