RPTOR抗体,Mouse Monoclonal RPTOR Antibody
  • RPTOR抗体,Mouse Monoclonal RPTOR Antibody
  • RPTOR抗体,Mouse Monoclonal RPTOR Antibody
  • RPTOR抗体,Mouse Monoclonal RPTOR Antibody

RPTOR抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-10
QQ交谈 微信洽谈

产品详情

中文名称:RPTOR抗体英文名称:Mouse Monoclonal RPTOR Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 666 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: RPTOR
2025-05-10 RPTOR抗体 Mouse Monoclonal RPTOR Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 666 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesKOG1; Mip1
Entrez GeneID57521
clone5A3A4
WB Predicted band size149kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human RPTOR (AA: 874-1009) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide.

+ +

  •  

     

       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


  •  

     

       Immunofluorescence analysis of HepG2 cells using RPTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)    


  •  

     

       Immunohistochemical analysis of paraffin-embedded human muscle tissues using RPTOR mouse mAb with DAB staining.    


           

参考文献

以下是3-4篇关于RPTOR抗体的参考文献,按文献名称、作者和摘要内容概括整理:

1. **文献名称**:*Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton*

**作者**:Sarbassov, D.D. et al. (2004)

**摘要**:研究首次发现RPTOR(raptor)与mTOR形成复合物(mTORC1),并利用特异性抗体验证其在调控细胞生长中的作用。抗体用于免疫共沉淀实验,揭示RPTOR在营养信号传导中的关键功能。

2. **文献名称**:*PRAS40 regulates mTORC1 kinase activity by functioning as a direct inhibitor of substrate binding*

**作者**:Sancak, Y. et al. (2007)

**摘要**:通过RPTOR抗体进行免疫印迹(Western blot)和免疫荧光分析,证明PRAS40通过结合RPTOR抑制mTORC1活性,揭示其在能量应激条件下的调控机制。

3. **文献名称**:*Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action in cells*

**作者**:Hara, K. et al. (2002)

**摘要**:早期研究利用RPTOR抗体验证其在mTOR复合物中的结构作用,发现RPTOR缺失导致mTOR底物(如S6K1)磷酸化水平下降,证实其对mTORC1功能不可或缺。

4. **文献名称**:*mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery*

**作者**:Kim, D.H. et al. (2002)

**摘要**:通过RPTOR特异性抗体进行免疫沉淀实验,证明RPTOR-mTOR复合物对氨基酸和生长因子敏感,调控蛋白质合成和细胞增殖。

这些文献均通过RPTOR抗体在分子机制研究中验证其功能,涵盖免疫沉淀、Western blot和复合物分析等实验方法。

       

背景信息

The RPTOR (Regulatory-Associated Protein of mTOR) antibody is a crucial tool in studying the mechanistic target of rapamycin (mTOR) signaling pathway, which regulates cell growth, proliferation, and metabolism. RPTOR, a core component of mTOR complex 1 (mTORC1), acts as a scaffold protein that recruits substrates and facilitates mTORC1 activation in response to nutrients, growth factors, and cellular energy status. Dysregulation of RPTOR-mediated mTORC1 signaling is implicated in cancer, metabolic disorders, and aging.

The RPTOR antibody enables researchers to detect and quantify RPTOR protein expression levels, assess its post-translational modifications (e.g., phosphorylation), and investigate its subcellular localization. It is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence. Specific RPTOR antibodies are validated for species cross-reactivity (e.g., human, mouse, rat) and tested for specificity using knockout controls to minimize off-target signals.

In research, this antibody aids in exploring mTORC1's role in diseases, drug discovery (e.g., mTOR inhibitors), and mechanistic studies of nutrient-sensing pathways. For example, it helps evaluate RPTOR expression changes under conditions like starvation, growth factor stimulation, or pharmacological inhibition. Its application extends to cancer biology, where mTORC1 hyperactivation is common, and to metabolic studies linking RPTOR to insulin signaling and autophagy regulation. Validation data, including molecular weight confirmation (~150 kDa) and functional assays, are critical for ensuring experimental reliability.

       
关键字: RPTOR抗体;RPTOR;RPTOR Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
普通会员
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

店内推荐

RPTOR抗体相关厂家报价

产品名称 价格   公司名称 报价日期
¥1785
VIP1年
上海信裕生物科技有限公司
2026-03-23
¥1710
VIP1年
上海钰博生物科技有限公司
2026-03-26
¥1800
VIP2年
费雪(杭州)医学研究有限公司
2026-03-27
内容声明:
以上所展示的信息由商家自行提供,内容的真实性、准确性和合法性由发布商家负责。 商家发布价格指该商品的参考价格,并非原价,该价格可能随着市场变化,或是由于您购买数量不同或所选规格不同而发生变化。最终成交价格,请咨询商家,以实际成交价格为准。请意识到互联网交易中的风险是客观存在的
主页 | 企业会员服务 | 广告业务 | 联系我们 | 旧版入口 | 中文MSDS | CAS Index | 常用化学品CAS列表 | 化工产品目录 | 新产品列表 |投诉中心
Copyright © 2008 ChemicalBook 京ICP备07040585号  京公海网安备110108000080号  All rights reserved.