The TIP60 (Tat-interactive protein 60) antibody is a crucial tool for studying the function and regulation of the TIP60 protein, a member of the MYST family of histone acetyltransferases (HATs). TIP60 plays a pivotal role in chromatin remodeling, DNA repair, cell cycle regulation, and apoptosis by acetylating histones (e.g., H4 and H2A) and non-histone targets, thereby modulating gene expression and protein interactions. It is also involved in critical signaling pathways, including the DNA damage response, where it interacts with tumor suppressors like p53.
TIP60 antibodies are widely used in research to detect and quantify TIP60 expression via techniques such as Western blotting, immunoprecipitation, immunofluorescence, and chromatin immunoprecipitation (ChIP). These antibodies are available in monoclonal and polyclonal forms, often raised in hosts like rabbits or mice, with specificity validated through knockout controls or competitive assays. Researchers rely on TIP60 antibodies to investigate its role in cancer (where it can act as both oncogene and tumor suppressor), epigenetic regulation, and cellular stress responses.
Due to its diverse functions, TIP60 antibody specificity is critical, as cross-reactivity with other MYST family members may occur. Proper validation ensures accurate interpretation in studies exploring TIP60's involvement in diseases or its therapeutic targeting.