Rab3a抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Flow cytometric analysis of HeLa cells using Rab3a mouse mAb (green) and negative control (red).    

  
  

Rab3a antibody is a widely used tool in neuroscience and cell biology research to detect and study the Rab3a protein, a member of the Rab GTPase family involved in regulating synaptic vesicle trafficking and neurotransmitter release. Rab3a plays a critical role in exocytosis, particularly in priming and docking synaptic vesicles to the presynaptic membrane prior to fusion. The antibody is designed to recognize specific epitopes of Rab3a, enabling its visualization and quantification in various experimental setups, including Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP).

Most Rab3a antibodies are raised against synthetic peptides or recombinant proteins corresponding to conserved regions of the protein, such as the N-terminal or C-terminal domains. They are available as monoclonal or polyclonal formats, with species reactivity typically covering humans, mice, and rats. Validation often involves knockout controls or siRNA-mediated silencing to confirm specificity, as cross-reactivity with other Rab isoforms (e.g., Rab3b, Rab3c) must be ruled out.

Research applications focus on understanding Rab3a's involvement in neurological disorders, including Alzheimer's disease, Parkinson's disease, and synaptic plasticity deficits. Commercial suppliers (e.g., Abcam, Santa Cruz Biotechnology, Cell Signaling Technology) provide Rab3a antibodies with detailed protocols and citations from peer-reviewed studies. Proper storage and dilution optimization are critical to maintain antibody performance. Its utility in mapping synaptic protein networks underscores its importance in neurobiological research.