FBXL10抗体,Mouse Monoclonal FBXL10 Antibody
  • FBXL10抗体,Mouse Monoclonal FBXL10 Antibody
  • FBXL10抗体,Mouse Monoclonal FBXL10 Antibody
  • FBXL10抗体,Mouse Monoclonal FBXL10 Antibody

FBXL10抗体 新品

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-10
QQ交谈 微信洽谈

产品详情

中文名称:FBXL10抗体英文名称:Mouse Monoclonal FBXL10 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 807 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: FBXL10
2025-05-10 FBXL10抗体 Mouse Monoclonal FBXL10 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 807 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC咨询技术 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesCXXC2; Fbl10; PCCX2; FBXL10; JHDM1B
Entrez GeneID84678
clone3B7D11
WB Predicted band size152.6kDa
Host/IsotypeMouse IgG2b
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human FBXL10 (AA: 457-555) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


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       Flow cytometric analysis of HeLa cells using FBXL10 mouse mAb (green) and negative control (red).    


           

参考文献

以下是关于FBXL10抗体的3篇参考文献示例(文献信息为虚拟概括,仅作参考):

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1. **文献名称**:*FBXL10 regulates polycomb-mediated chromatin modifications through ubiquitination in embryonic stem cells*

**作者**:He J, et al.

**摘要**:本研究利用FBXL10特异性抗体进行染色质免疫沉淀(ChIP),揭示了FBXL10通过招募Cullin-RING泛素连接酶复合物,调控多梳蛋白(Polycomb)介导的组蛋白H2A泛素化,从而维持胚胎干细胞的自我更新能力。

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2. **文献名称**:*KDM2B/FBXL10 demethylates H3K36me3 to facilitate cellular senescence*

**作者**:Frescas D, et al.

**摘要**:通过FBXL10抗体的Western blot和免疫荧光实验,研究者发现FBXL10通过去甲基化H3K36me3修饰,激活衰老相关基因表达,并证实其在细胞衰老过程中的表观遗传调控作用。

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3. **文献名称**:*FBXL10 interacts with p53 to suppress tumorigenesis via modulating ribosomal RNA transcription*

**作者**:Blackledge NP, et al.

**摘要**:使用FBXL10抗体进行免疫共沉淀(Co-IP)和蛋白质互作分析,证明FBXL10与肿瘤抑制蛋白p53直接结合,共同抑制核糖体RNA的转录,从而抑制肿瘤发生。

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4. **文献名称**:*Structural and functional analysis of the FBXL10-SKP1 complex in chromatin remodeling*

**作者**:Tsukada Y, et al.

**摘要**:通过FBXL10抗体的体外Pull-down实验,解析了FBXL10与SKP1蛋白的相互作用结构域,并阐明其通过SCF泛素化复合物调控染色质重塑的分子机制。

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(注:上述文献为示例性概括,实际引用时需核实真实文献信息。)

       

背景信息

FBXL10 (F-box and leucine-rich repeat protein 10), also known as JHDM1B or KDM2B, is a member of the F-box protein family that plays a role in ubiquitin-mediated proteolysis by forming SCF (Skp1-Cul1-F-box) E3 ubiquitin ligase complexes. It contains an F-box domain for Skp1 binding and leucine-rich repeats (LRRs) for substrate interaction, enabling substrate recognition and degradation. Additionally, FBXL10 functions as a histone demethylase, targeting H3K36me2/me1 through its JmjC domain, thereby regulating gene expression and epigenetic silencing.

FBXL10 antibodies are essential tools for studying its dual role in protein ubiquitination and chromatin remodeling. These antibodies are used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect FBXL10 expression, localization, and interactions. Researchers employ them to explore FBXL10's involvement in cellular processes such as cell cycle regulation, differentiation, and tumorigenesis. Studies have linked FBXL10 to both oncogenic and tumor-suppressive activities, depending on cellular context, making it a focus in cancer research.

Antibody specificity is critical due to FBXL10’s splice variants (e.g., FBXL10-1 and FBXL10-2) and post-translational modifications. Validated antibodies help distinguish its roles in recruiting Polycomb repressive complexes or degrading substrates like p53. FBXL10 antibodies also aid in dissecting its tissue-specific functions, particularly in stem cells and developmental models. Overall, these antibodies are vital for unraveling FBXL10's complex regulatory networks in health and disease.

       
关键字: FBXL10抗体;FBXL10;FBXL10 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

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