The YWHAB antibody is a tool used to detect and study the YWHAB protein, a member of the 14-3-3 family of conserved regulatory proteins. These proteins play critical roles in signal transduction, cell cycle regulation, apoptosis, and stress responses by binding to phosphorylated serine/threonine residues on target proteins. YWHAB (14-3-3β) is ubiquitously expressed and involved in diverse cellular processes, including neuronal development, cancer progression, and metabolic regulation. It acts as a molecular scaffold, modulating interactions between signaling molecules such as kinases, phosphatases, and transcription factors.
Antibodies targeting YWHAB are essential for investigating its expression, localization, and function in both physiological and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein levels in tissues or cells. Dysregulation of YWHAB has been linked to diseases such as cancer, neurodegenerative disorders (e.g., Alzheimer’s), and metabolic syndromes, making its study clinically relevant.
Commercial YWHAB antibodies are typically monoclonal or polyclonal, validated for specificity and cross-reactivity across species (human, mouse, rat). Researchers rely on these antibodies to explore YWHAB’s role in signaling pathways, its interaction partners, and potential as a therapeutic target. However, challenges like batch variability or cross-reactivity with other 14-3-3 isoforms (e.g., YWHAZ) require careful validation in experimental setups.