ROP1抗体

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Western blot analysis using ROP1 mouse mAb against BCBL-1 (1) and Raw264.7 (2) cell lysate.    

  
  

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     Flow cytometric analysis of MCF-7 cells using ROP1 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using ROP1 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using ROP1 mouse mAb with DAB staining.    

  
  

The ROP1 antibody targets the rhoptry protein 1 (ROP1) of *Toxoplasma gondii*, an obligate intracellular parasite responsible for toxoplasmosis. Rhoptries are specialized secretory organelles in apicomplexan parasites, critical for host cell invasion and establishing intracellular niches. ROP1. a major component of rhoptry necks, is secreted during parasite entry and contributes to host membrane penetration and parasitophorous vacuole formation. Although its exact molecular role remains debated, ROP1 is thought to interact with host cell components to facilitate invasion.

ROP1 antibodies, often generated in mice or rabbits via recombinant protein immunization, are widely used in research to study rhoptry function, parasite invasion mechanisms, and host-pathogen interactions. They enable detection of ROP1 expression via techniques like Western blotting, immunofluorescence, and immunohistochemistry, aiding in visualizing rhoptry morphology and protein localization. Additionally, ROP1 antibodies have diagnostic potential, helping identify active *T. gondii* infections in clinical samples. Their utility extends to evaluating vaccine candidates targeting rhoptry proteins, as inhibiting ROP1 could block parasitic entry. Despite being a historical marker for rhoptries, recent studies suggest ROP1 may not be essential for invasion in all strains, highlighting functional redundancy in rhoptry proteins. Overall, ROP1 antibodies remain vital tools for advancing toxoplasmosis research and therapeutic development.