ASH2L抗体,Mouse Monoclonal ASH2L Antibody
  • ASH2L抗体,Mouse Monoclonal ASH2L Antibody
  • ASH2L抗体,Mouse Monoclonal ASH2L Antibody
  • ASH2L抗体,Mouse Monoclonal ASH2L Antibody

ASH2L抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-10
QQ交谈 微信洽谈

产品详情

中文名称:ASH2L抗体英文名称:Mouse Monoclonal ASH2L Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 934 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: ASH2L
2025-05-10 ASH2L抗体 Mouse Monoclonal ASH2L Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 934 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesASH2; Bre2; ASH2L1; ASH2L2
Entrez GeneID9070
clone6F6B9
WB Predicted band size68.7kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenPurified recombinant fragment of human ASH2L (AA: 493-628) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

+ +

  •  

     

       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


  •  

     

       Western blot analysis using ASH2L mouse mAb against K562 (1) and F9 (2) cell lysate.    


  •  

     

       Flow cytometric analysis of K562 cells using ASH2L mouse mAb (green) and negative control (red).    


  •  

     

       Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using ASH2L mouse mAb with DAB staining.    


  •  

     

       Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using ASH2L mouse mAb with DAB staining.    


           

参考文献

以下是关于ASH2L抗体的参考文献示例(注:以下内容为示例性概括,具体文献需通过学术数据库核实):

---

1. **文献名称**: "ASH2L is essential for the histone H3K4 methylation maintenance in acute leukemia"

**作者**: Johnson M, et al.

**摘要**: 本研究利用ASH2L抗体进行染色质免疫沉淀(ChIP),揭示了ASH2L在维持白血病细胞H3K4三甲基化中的关键作用,其缺失导致HOX基因表达下调并抑制癌细胞增殖。

2. **文献名称**: "Structural basis of ASH2L recognition by the MLL complex in epigenetic regulation"

**作者**: Li X, et al.

**摘要**: 通过ASH2L抗体介导的免疫共沉淀和冷冻电镜技术,解析了ASH2L与MLL复合体的相互作用机制,阐明其在H3K4甲基化及基因激活中的结构基础。

3. **文献名称**: "ASH2L promotes hepatocellular carcinoma progression via modulating ERK signaling pathway"

**作者**: Wang Q, et al.

**摘要**: 研究使用ASH2L抗体进行免疫组化分析,发现ASH2L在肝癌中高表达,并通过调控ERK信号通路促进肿瘤侵袭,提示其作为潜在治疗靶点。

4. **文献名称**: "ASH2L deficiency disrupts embryonic stem cell differentiation by altering histone modification landscapes"

**作者**: Kim S, et al.

**摘要**: 结合ASH2L抗体的功能缺失实验,证明ASH2L通过维持H3K4me3水平调控胚胎干细胞多能性基因,其缺失导致分化异常及胚胎发育缺陷。

---

建议通过**PubMed**或**Google Scholar**检索关键词“ASH2L antibody”或“ASH2L function”获取最新文献。实际研究中,ASH2L常与表观遗传调控、癌症及发育疾病相关。

       

背景信息

ASH2L (Absent Small and Homeotic Disks 2 Homolog) is a core component of the multi-protein COMPASS (Complex of Proteins Associated with Set1) family, which plays a critical role in regulating histone H3 lysine 4 methylation (H3K4me), a hallmark of transcriptionally active chromatin. ASH2L functions as a scaffolding protein that stabilizes interactions within the COMPASS complex, enabling the enzymatic subunits (e.g., MLL1-4. SET1A/B) to catalyze mono-, di-, or trimethylation of H3K4. This epigenetic modification is essential for maintaining proper gene expression patterns during development, differentiation, and cellular homeostasis.

ASH2L antibodies are widely used in research to study its role in chromatin remodeling, transcriptional regulation, and disease mechanisms. They are employed in techniques like chromatin immunoprecipitation (ChIP-seq), Western blotting, and immunofluorescence to map ASH2L binding sites, assess protein expression levels, or visualize its nuclear localization. Dysregulation of ASH2L has been linked to cancers (e.g., leukemia, breast cancer) and developmental disorders, as aberrant H3K4 methylation can disrupt normal gene activation. ASH2L antibodies also help investigate its interactions with other COMPASS components (e.g., WDR5. RbBP5) and explore therapeutic strategies targeting epigenetic pathways. Both polyclonal and monoclonal antibodies are available, often validated for specificity using knockout cell lines or siRNA knockdowns.

       
关键字: ASH2L抗体;ASH2L;ASH2L Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

店内推荐

ASH2L抗体相关厂家报价

产品名称 价格   公司名称 报价日期
¥1280
湖北艾普蒂生物工程有限公司
2025-06-04
询价
碧云天生物技术有限公司
2025-12-28
询价
VIP4年
维百奥(北京)生物科技有限公司
2025-12-29
内容声明:
以上所展示的信息由商家自行提供,内容的真实性、准确性和合法性由发布商家负责。 商家发布价格指该商品的参考价格,并非原价,该价格可能随着市场变化,或是由于您购买数量不同或所选规格不同而发生变化。最终成交价格,请咨询商家,以实际成交价格为准。请意识到互联网交易中的风险是客观存在的
主页 | 企业会员服务 | 广告业务 | 联系我们 | 旧版入口 | 中文MSDS | CAS Index | 常用化学品CAS列表 | 化工产品目录 | 新产品列表 |投诉中心
Copyright © 2008 ChemicalBook 京ICP备07040585号  京公海网安备110108000080号  All rights reserved.