DUSP6抗体,Rabbit Polyclonal DUSP6 Antibody
  • DUSP6抗体,Rabbit Polyclonal DUSP6 Antibody
  • DUSP6抗体,Rabbit Polyclonal DUSP6 Antibody
  • DUSP6抗体,Rabbit Polyclonal DUSP6 Antibody

DUSP6抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-11
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产品详情

中文名称:DUSP6抗体英文名称:Rabbit Polyclonal DUSP6 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 1676 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: DUSP6
2025-05-11 DUSP6抗体 Rabbit Polyclonal DUSP6 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 1676 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/25-1/100 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/1000-1/5000 Human,Mouse,Rat
   

产品详情

AliasesMKP3, PYST1
WB Predicted band size27 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenFusion protein of human DUSP6
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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       Gel: 10%SDS-PAGE, Lysate: 30 μg, Lane 1-2: Jurkat cells, human fetal kidney tissue, Primary antibody: P00359(DUSP6 Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    


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       The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using P00359(DUSP6 Antibody) at dilution 1/25. (Original magnification: ×200)    


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       The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P00359(DUSP6 Antibody) at dilution 1/25. (Original magnification: ×200)    


           

参考文献

以下是关于DUSP6抗体的3篇参考文献,格式包含文献名称、作者及简要摘要内容:

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1. **文献名称**: *DUSP6/MKP-3 inactivates ERK1/2 but fails to bind and inactivate ERK5*

**作者**: Mandl M, Slack DN, Keyse SM

**摘要**: 研究验证了DUSP6对ERK1/2的去磷酸化作用,并利用特异性抗体通过Western blot分析其在HEK293细胞中的表达,揭示了DUSP6对ERK5无调控作用的机制。

2. **文献名称**: *DUSP6 is a novel transcriptional target of p53 and regulates chemosensitivity*

**作者**: Li JP, Yang CY, Chuang HC, et al.

**摘要**: 通过免疫组化(DUSP6抗体)和基因沉默技术,发现p53通过调控DUSP6表达影响癌细胞对化疗药物的敏感性,为肿瘤治疗提供潜在靶点。

3. **文献名称**: *DUSP6 deficiency prevents endothelial dysfunction and attenuates atherosclerosis in mice*

**作者**: Yokoyama S, Mori K, Nagamori S, et al.

**摘要**: 研究利用DUSP6敲除小鼠及抗体检测(免疫荧光),证明DUSP6通过MAPK通路调控血管内皮功能,其缺失可减缓动脉粥样硬化进程。

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以上文献均涉及DUSP6抗体的实验应用(如Western blot、免疫组化等),并探讨其在信号通路、癌症或心血管疾病中的功能。如需更多细节或近年研究,可进一步筛选特定数据库(如PubMed)。

       

背景信息

DUSP6 (dual-specificity phosphatase 6), also known as MKP-3 (mitogen-activated protein kinase phosphatase 3), is a member of the dual-specificity phosphatase family that regulates mitogen-activated protein kinase (MAPK) signaling pathways. It specifically dephosphorylates and inactivates extracellular signal-regulated kinases 1 and 2 (ERK1/2), critical components of the RAS/RAF/MEK/ERK cascade involved in cell proliferation, differentiation, and apoptosis. DUSP6 acts as a negative feedback regulator, ensuring tight control of ERK activity to maintain cellular homeostasis. Dysregulation of DUSP6 expression or function has been implicated in various diseases, including cancer, diabetes, and cardiovascular disorders, making it a focus of therapeutic research.

Antibodies targeting DUSP6 are essential tools for studying its expression, localization, and role in signaling pathways. They enable detection of endogenous DUSP6 protein levels in tissues or cultured cells via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies are also used to investigate DUSP6's interaction with ERK1/2 and its modulation under physiological or pathological conditions, such as oncogenic stress or metabolic dysfunction. Specificity is critical, as cross-reactivity with other DUSP family members (e.g., DUSP5 or DUSP7) can occur. Validated DUSP6 antibodies help explore its potential as a biomarker or therapeutic target, particularly in cancers where ERK signaling is hyperactive. Researchers often verify antibody performance using knockout controls or siRNA-mediated knockdown to ensure reliability in experimental models.

       
关键字: DUSP6抗体;DUSP6;DUSP6 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
普通会员
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

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