PHF6 (Plant Homeodomain Finger Protein 6) is a chromatin-associated protein encoded by the *PHF6* gene located on the X chromosome. It contains two plant-homeodomain (PHD) zinc finger motifs, which are critical for interactions with histone modifications and chromatin remodeling complexes. PHF6 plays a role in transcriptional regulation, cell cycle control, and neural development. Mutations in *PHF6* are linked to genetic disorders such as Börjeson-Forssman-Lehmann syndrome (BFLS), characterized by intellectual disability, obesity, and facial dysmorphism, and are also implicated in hematologic malignancies, particularly T-cell acute lymphoblastic leukemia (T-ALL).
PHF6 antibodies are essential tools for studying the protein's expression, localization, and function in both normal and disease contexts. These antibodies enable detection of PHF6 in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers use PHF6-specific antibodies to explore its role in chromatin dynamics, its interaction with nucleolar proteins like nucleophosmin, and its tumor-suppressive mechanisms in leukemia. Given PHF6's tissue-specific expression patterns and its involvement in developmental and oncogenic pathways, high-quality antibodies are crucial for dissecting molecular pathways and validating clinical correlations in PHF6-related diseases. Validation of antibody specificity, including knockout cell line controls, is critical to ensure reliable experimental outcomes.