The PLEKHO1 (Pleckstrin Homology Domain-Containing Family O Member 1) antibody is a tool used to study the PLEKHO1 protein, also known as CKIP-1 (Casein Kinase 2-Interacting Protein 1). This protein plays a regulatory role in multiple cellular processes, including cell proliferation, apoptosis, cytoskeletal organization, and bone formation. It interacts with signaling pathways such as the PI3K/AKT and Wnt/β-catenin cascades, often acting as a scaffold or adaptor protein. PLEKHO1 is implicated in diseases like cancer, osteoporosis, and cardiovascular disorders, making it a target for therapeutic research.
The antibody is widely utilized in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect PLEKHO1 expression, localization, and interactions in cells or tissues. Studies using this antibody have revealed PLEKHO1’s role in tumor suppression (e.g., inhibiting lung cancer metastasis) or promotion (context-dependent), bone metabolism (modulating osteoblast activity), and cardiac remodeling. Its development often involves immunizing hosts with peptide antigens specific to conserved regions of the human or murine PLEKHO1 protein, followed by purification and validation for specificity. Researchers rely on this antibody to explore PLEKHO1’s molecular mechanisms, potential as a biomarker, or as a target for drug development in precision medicine.