The CPSF4 antibody is a tool used to detect the Cleavage and Polyadenylation Specificity Factor 4 (CPSF4), a critical subunit of the CPSF complex involved in mRNA processing. CPSF4. also known as CPSF30. plays a key role in the cleavage and polyadenylation of pre-mRNAs during transcription termination, ensuring proper 3ʹ-end formation and stability of mRNA. This process is essential for gene expression regulation, and dysregulation of CPSF4 has been linked to cellular differentiation, immune responses, viral infections, and diseases like cancer.
CPSF4 antibodies are typically produced in hosts such as rabbits or mice using immunogenic peptides or recombinant CPSF4 proteins. These antibodies enable researchers to study CPSF4's expression, localization, and interactions via techniques like Western blotting, immunoprecipitation, and immunofluorescence. They are valuable in exploring CPSF4's role in viral replication, as some viruses (e.g., influenza) exploit CPSF4 to suppress host mRNA processing. Additionally, studies use these antibodies to investigate CPSF4's involvement in cancer progression, stem cell biology, and neurological disorders.
Validated for specificity through knockout controls or siRNA knockdowns, CPSF4 antibodies are crucial for elucidating molecular mechanisms in RNA metabolism and disease pathways. Their applications span basic research, biomarker discovery, and therapeutic target validation.