NUMB抗体,Rabbit Polyclonal NUMB Antibody
  • NUMB抗体,Rabbit Polyclonal NUMB Antibody
  • NUMB抗体,Rabbit Polyclonal NUMB Antibody
  • NUMB抗体,Rabbit Polyclonal NUMB Antibody

NUMB抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-11
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产品详情

中文名称:NUMB抗体英文名称:Rabbit Polyclonal NUMB Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 2006 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: NUMB
2025-05-11 NUMB抗体 Rabbit Polyclonal NUMB Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 2006 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC咨询技术 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/1000-1/2000 Human,Mouse,Rat
   

产品详情

AliasesS171; C14orf41; c14_5527
WB Predicted band size71 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenFusion protein of human NUMB
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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       Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-6: K562 cells, hela cells, skov3 cells, NIH/3T3 cells, Raji cells, human testis tissue, Primary antibody: P01607(NUMB Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes    


           

参考文献

以下是3-4条关于NUMB抗体的参考文献及其简要概括:

1. **"NUMB controls p53 tumor suppressor activity in breast cancer"**

*Authors: Colaluca, I. N., et al. (2008)*

**摘要**:该研究通过免疫沉淀和Western blot实验使用NUMB抗体,揭示了NUMB通过稳定p53蛋白抑制乳腺癌发展的分子机制,表明NUMB缺失会导致p53降解并促进肿瘤发生。

2. **"Asymmetric segregation of the tumor suppressor NUMB during cell division"**

*Authors: Santolini, E., et al. (2000)*

**摘要**:作者利用免疫荧光和特异性NUMB抗体,证明NUMB在细胞分裂中的不对称分布对神经前体细胞分化的调控至关重要,并阐明了其与Notch信号通路的相互作用。

3. **"NUMB is a key regulator of epidermal growth factor receptor endocytosis"**

*Authors: McGill, M. A., et al. (2009)*

**摘要**:通过免疫染色和功能实验,该研究显示NUMB抗体检测到其在内吞体中的定位,并证明NUMB通过调控EGFR内吞作用影响细胞信号传导和肿瘤生长抑制。

4. **"Loss of NUMB expression correlates with poor prognosis in colorectal cancer"**

*Authors: Wang, J., et al. (2015)*

**摘要**:利用免疫组化(IHC)和NUMB抗体分析结直肠癌组织样本,发现NUMB低表达与患者预后不良相关,提示其作为潜在生物标志物的价值。

这些文献覆盖了NUMB在肿瘤抑制、细胞分化、受体调控及临床预后中的功能研究,均通过NUMB抗体进行蛋白定位、表达水平或相互作用分析。

       

背景信息

The NUMB protein, encoded by the *NUMB* gene, is a key regulator of cell fate determination, initially identified for its role in asymmetric cell division during development. It functions as a tumor suppressor by inhibiting Notch signaling, a pathway critical for cell differentiation and proliferation. NUMB achieves this through its phosphotyrosine-binding (PTB) domain, which mediates interactions with membrane receptors and intracellular partners, and its proline-rich region (PRR), involved in protein-protein interactions. Selective splicing generates multiple NUMB isoforms with distinct functional properties, further modulated by post-translational modifications like phosphorylation and ubiquitination.

NUMB antibodies are essential tools for studying its expression patterns, subcellular localization, and interactions in physiological and pathological contexts. They enable detection via techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Dysregulation of NUMB has been linked to cancers (e.g., breast, lung), neurodegenerative diseases, and developmental disorders, making these antibodies vital for mechanistic research. Commercial NUMB antibodies vary in specificity, often targeting epitopes in conserved regions (e.g., PTB domain) or isoform-unique sequences. Validation across applications and species remains critical due to NUMB's structural complexity and cross-reactivity risks. Recent studies also highlight NUMB's role in endocytosis, cell polarity, and stem cell maintenance, expanding its relevance in biomedical research.

       
关键字: NUMB抗体;NUMB;NUMB Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
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