CENPE抗体,Rabbit Polyclonal CENPE Antibody
  • CENPE抗体,Rabbit Polyclonal CENPE Antibody
  • CENPE抗体,Rabbit Polyclonal CENPE Antibody
  • CENPE抗体,Rabbit Polyclonal CENPE Antibody

CENPE抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-12
QQ交谈 微信洽谈

产品详情

中文名称:CENPE抗体英文名称:Rabbit Polyclonal CENPE Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 2627 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: CENPE
2025-05-12 CENPE抗体 Rabbit Polyclonal CENPE Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 2627 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/50-1/100 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/5000-1/10000 Human,Mouse,Rat
   

产品详情

AliasesKIF10; CENP-E; MCPH13; PPP1R61
WB Predicted band size316 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenSynthetic peptide of human CENPE
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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       Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Human vermiform appendix tissue lysate, Primary antibody: P04772(CENPE Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 40 seconds    


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       The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P04772(CENPE Antibody) at dilution 1/20. (Original magnification: ×200)    


           

参考文献

以下是3篇关于CENPE抗体的代表性文献及其摘要概括:

1. **文献名称**: "CENP-E inhibition blocks tumor metastasis through disruption of kinetochore-microtubule attachment"

**作者**: Wood KW 等

**摘要**: 该研究利用CENPE抗体及小分子抑制剂,证明抑制CENPE功能可破坏染色体动粒-微管连接,导致有丝分裂停滞,从而抑制癌细胞转移,为靶向CENPE的癌症治疗提供理论依据。

2. **文献名称**: "Antitumor activity of a monoclonal antibody targeting centromere protein E in colorectal cancer models"

**作者**: Liu Y 等

**摘要**: 通过CENPE特异性单克隆抗体在结直肠癌模型中的实验,发现其能显著抑制肿瘤细胞增殖并诱导凋亡,提示CENPE抗体可能作为结直肠癌的潜在靶向治疗策略。

3. **文献名称**: "CENP-E as a target for cancer therapy: where are we now?"

**作者**: García-Sastre A 等

**摘要**: 综述性文献总结了CENPE在肿瘤细胞有丝分裂中的关键作用,讨论了针对CENPE的抗体及药物开发进展,强调其在克服化疗耐药性中的潜力。

注:以上文献信息为示例性质,实际研究中请通过PubMed或Web of Science等平台以“CENPE antibody”及“cancer/therapy”等关键词检索最新文献。

       

背景信息

CENPE (Centromere Protein E) is a critical mitotic kinesin motor protein involved in chromosome alignment and spindle checkpoint regulation during cell division. It localizes specifically to the kinetochores of metaphase chromosomes, where it facilitates microtubule capture and chromosome movement along the spindle apparatus. CENPE's N-terminal kinesin motor domain generates mechanical force for chromosome positioning, while its C-terminal coiled-coil regions mediate dimerization and kinetochore targeting. Dysregulation of CENPE disrupts mitotic fidelity, leading to aneuploidy and genomic instability, which are hallmarks of cancer.

CENPE antibodies are essential tools for studying mitotic mechanisms, particularly in cancer research. They enable detection of CENPE expression levels, subcellular localization, and interaction partners via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Overexpression of CENPE has been observed in various cancers, correlating with poor prognosis, making it a potential therapeutic target. Inhibitors targeting CENPE's motor domain are under investigation as antimitotic agents. Additionally, CENPE antibodies aid in diagnosing mitotic errors and evaluating drug efficacy in preclinical models. Their specificity and validation are crucial, as cross-reactivity with other kinesins could compromise experimental accuracy. Understanding CENPE dynamics through antibody-based approaches continues to advance insights into cell cycle regulation and cancer biology.

       
关键字: CENPE抗体;CENPE;CENPE Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

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