CDC25A抗体,Rabbit Polyclonal CDC25A Antibody
  • CDC25A抗体,Rabbit Polyclonal CDC25A Antibody
  • CDC25A抗体,Rabbit Polyclonal CDC25A Antibody

CDC25A抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-12
QQ交谈 微信洽谈

产品详情

中文名称:CDC25A抗体英文名称:Rabbit Polyclonal CDC25A Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 3413 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: CDC25A
2025-05-12 CDC25A抗体 Rabbit Polyclonal CDC25A Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 3413 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/50-1/200 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/5000-1/10000 Human,Mouse,Rat
   

产品详情

AliasesCDC25A2
WB Predicted band size59 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenFusion protein of human CDC25A
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

+ +

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       Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse heart tissue lysate, Primary antibody: P07179(CDC25A Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 10 seconds    


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       The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P07179(CDC25A Antibody) at dilution 1/75. (Original magnification: ×200)    


           

参考文献

以下是关于CDC25A抗体的参考文献示例(内容为虚构,仅作格式参考):

1. **"CDC25A phosphatase as a biomarker in colorectal cancer: Antibody-based detection and clinical implications"**

*Authors: J. Smith et al.*

摘要:本研究利用特异性CDC25A抗体,通过免疫组化分析结直肠癌组织,发现CDC25A高表达与患者生存率降低显著相关,提示其作为预后标志物的潜力。

2. **"Development of a monoclonal antibody targeting CDC25A for cell cycle regulation studies"**

*Authors: L. Chen & M. Tanaka*

摘要:报道了一种新型抗CDC25A单克隆抗体的开发,验证其在Western blot和免疫荧光中的特异性,并用于探究CDC25A在G1/S期检查点中的功能调控。

3. **"Ubiquitin-mediated degradation of CDC25A: Insights from siRNA and antibody inhibition experiments"**

*Authors: R. Gupta et al.*

摘要:结合RNA干扰和CDC25A抗体阻断实验,揭示了泛素-蛋白酶体系统对CDC25A的降解机制,阐明其在DNA损伤应答中的动态调控。

4. **"CDC25A overexpression in triple-negative breast cancer: Therapeutic target validation via neutralizing antibodies"**

*Authors: E. Martínez et al.*

摘要:通过中和性CDC25A抗体抑制乳腺癌细胞增殖,证实靶向CDC25A可增强化疗敏感性,为三阴性乳腺癌治疗提供新策略。

(注:以上文献及作者为模拟示例,实际引用请查询PubMed等数据库获取真实研究。)

       

背景信息

CDC25A is a dual-specificity phosphatase belonging to the CDC25 family, which plays a critical role in regulating cell cycle progression. It primarily activates cyclin-dependent kinases (CDKs) by removing inhibitory phosphorylations, thereby driving transitions through the G1/S and G2/M checkpoints. Overexpression or dysregulation of CDC25A is linked to uncontrolled cell proliferation, genomic instability, and tumorigenesis, making it a biomarker of interest in cancers such as breast, lung, and colorectal malignancies.

Antibodies targeting CDC25A are essential tools for studying its expression, localization, and function in both normal and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to quantify protein levels or assess subcellular distribution. High-quality CDC25A antibodies are typically validated for specificity using knockout (KO) cell lines or siRNA-mediated knockdown to minimize cross-reactivity with homologous family members (e.g., CDC25B/C). Species reactivity often includes human, mouse, and rat samples.

In cancer research, CDC25A antibodies help evaluate its prognostic significance and therapeutic potential. Small-molecule inhibitors targeting CDC25A are under investigation, and these antibodies aid in mechanistic studies of drug efficacy. Challenges include distinguishing post-translational modifications (e.g., phosphorylation) that regulate CDC25A stability during DNA damage responses. Researchers must optimize experimental conditions to account for such dynamic changes, ensuring accurate interpretation of CDC25A-related pathways in health and disease.

       
关键字: CDC25A抗体;CDC25A;CDC25A Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
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