The RPL17 antibody is a research tool designed to detect ribosomal protein L17 (RPL17), a component of the 60S ribosomal subunit involved in protein synthesis. RPL17. part of the conserved ribosomal protein family, plays a critical role in ribosome assembly and translational fidelity. Its expression is essential for cellular homeostasis, with dysregulation linked to diseases such as cancer, developmental disorders, and ribosomopathies. The antibody is commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study RPL17's expression levels, subcellular localization, and interactions in various biological contexts.
RPL17 antibodies are typically raised in hosts like rabbits or mice using immunogenic peptides derived from specific regions of the protein (e.g., C-terminal or N-terminal epitopes). Validation includes knockout cell lines or siRNA-mediated silencing to confirm specificity. Researchers utilize these antibodies to explore RPL17's roles in cell proliferation, differentiation, and stress responses, as well as its potential as a biomarker in cancer due to its altered expression in tumors. Additionally, studies investigate its involvement in ribosomal stress pathways and crosstalk with p53-mediated signaling. Proper controls and optimization are critical for reliable results, given the ubiquitous expression of ribosomal proteins across tissues.