描述(Description)
IFNγ is the major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly byT-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFNγ synthesis is induced by IL-2, FGF-basic, and EGF.
纯度(Purity)
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
生物活性(Bioactivity)
Measured in a cell proliferation assay using Mo7e human leukemia cell line . The ED50 for this effect is ≤ 2 ng/mL.
氨基酸序列(Target Protein Sequence)
QDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRGRRASQ
制剂(Trait)
Lyophilized after extensive dialysis against PBS or PBS, pH 7.4, 8% trehalose
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存条件&效期(Storage & Stability)
Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
运输方式(Shipping)
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below

生物活性(Bioactivity)
Recombinant Human IFNγ stimulates proliferation of Mo7e cells. The ED50 for this effect is ≤2 ng/mL. TheBioactivity of Y01801 was higher than the other competing product.

电泳(SDS-PAGE)
Recombinant Human IFNγ SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y01801 SDS-PAGE
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关键字: GMP级;高纯度;批间一致;生物活性;科研专供;
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