CD63抗体,Mouse Monoclonal CD63 Antibody
  • CD63抗体,Mouse Monoclonal CD63 Antibody
  • CD63抗体,Mouse Monoclonal CD63 Antibody
  • CD63抗体,Mouse Monoclonal CD63 Antibody

CD63抗体—艾普蒂

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-05-15
QQ交谈 微信洽谈

产品详情

中文名称:CD63抗体英文名称:Mouse Monoclonal CD63 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 1066 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: CD63
2025-05-15 CD63抗体 Mouse Monoclonal CD63 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 1066 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesMLA1; ME491; LAMP-3; OMA81H; TSPAN30
Entrez GeneID967
clone1E8D4
WB Predicted band size25.6kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human CD63 (AA: extra 103-203) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Flow cytometric analysis of Hela cells using CD63 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using CD63 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using CD63 mouse mAb with DAB staining.    


           

参考文献

以下是3篇关于CD63抗体的代表性文献摘要:

1. **"Identification of CD63 as a tissue-specific marker of extracellular vesicles"**

- 作者:Thery, C., et al. (2006)

- 摘要:该研究通过流式细胞术和免疫电镜验证了CD63抗体作为外泌体特异性标记物的有效性,发现CD63在多种细胞来源的胞外囊泡表面高表达,为外泌体分离和表征提供了关键工具。

2. **"CD63 regulates cancer cell migration and stemness via integrin signaling"**

- 作者:Rana, S., et al. (2012)

- 摘要:利用CD63抗体阻断实验,揭示了CD63通过调控整合素β1的膜定位影响肿瘤细胞迁移和干性维持的机制,提示其作为癌症治疗潜在靶点。

3. **"Tetraspanin CD63 controls endosomal trafficking and extracellular vesicle biogenesis"**

- 作者:Pols, M.S., Klumperman, J. (2009)

- 摘要:通过CD63抗体介导的免疫沉淀结合共聚焦显微技术,证实CD63参与多泡体成熟和囊泡分泌过程,阐明其在细胞内运输和细胞间通讯中的核心作用。

4. **"Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes"**

- 作者:Kowal, J., et al. (2016)

- 摘要:通过CD63抗体与其他跨膜蛋白抗体的联合应用,建立了外泌体亚群分选的蛋白质组学方法,证明CD63可作为区分不同EV亚型的特异性标志物。

注:以上文献为领域内经典研究,如需具体DOI或期刊信息可进一步补充。实际引用时建议通过PubMed等平台核对最新版本。

       

背景信息

CD63 antibody is a tool used to detect CD63. a transmembrane protein belonging to the tetraspanin family. CD63. also known as lysosomal-associated membrane protein 3 (LAMP-3), is widely expressed in various cell types and is enriched in endosomal/lysosomal compartments and exosomes. It plays roles in cell adhesion, migration, intracellular trafficking, and signal transduction. As a marker of exosomes, CD63 is frequently employed in extracellular vesicle research to characterize these nanovesicles involved in intercellular communication, cancer progression, and immune regulation.

CD63 antibodies are crucial for identifying exosomes via techniques like flow cytometry, Western blotting, or immunofluorescence. They also aid in studying CD63's interaction with integrins, receptors, and other tetraspanins in membrane microdomains. Dysregulation of CD63 is linked to cancer metastasis, neurodegenerative diseases, and platelet activation. In diagnostics, CD63 antibodies may help profile disease-specific exosomes. Therapeutically, CD63 is explored for targeted drug delivery due to its exosomal surface presence.

Species cross-reactivity (human, mouse, rat) and epitope specificity vary among antibody clones, requiring validation for experimental contexts. Despite its utility, CD63's heterogeneous distribution across cell types and vesicles underscores the need for complementary markers in research.

       
关键字: CD63抗体;CD63;CD63 Antibody;

公司简介

是一家科研、开发、生产和销售为一体的科技型企业,
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,蛋白组学,细胞生物学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
普通会员
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:重组蛋白、抗体、生物试剂
  • 公司地址:青浦区
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