EHMT2抗体,Mouse Monoclonal EHMT2 Antibody
  • EHMT2抗体,Mouse Monoclonal EHMT2 Antibody
  • EHMT2抗体,Mouse Monoclonal EHMT2 Antibody
  • EHMT2抗体,Mouse Monoclonal EHMT2 Antibody

EHMT2抗体—艾普蒂

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-05-15
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产品详情

中文名称:EHMT2抗体英文名称:Mouse Monoclonal EHMT2 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 1190 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: EHMT2
2025-05-15 EHMT2抗体 Mouse Monoclonal EHMT2 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 1190 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesG9A; BAT8; GAT8; NG36; KMT1C; C6orf30
Entrez GeneID10919
clone2D12A11
WB Predicted band size132.4kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human EHMT2 (AA: 317-471) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Immunofluorescence analysis of Hela cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.    


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       Immunofluorescence analysis of Hela cells using EHMT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of HL-60 cells using EHMT2 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using EHMT2 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using EHMT2 mouse mAb with DAB staining.    


           

参考文献

以下是关于EHMT2(G9a)抗体的3篇代表性文献,涵盖其在不同研究中的应用及功能分析:

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1. **文献名称**:*EHMT2/G9a as a Therapeutic Target in Cancer*

**作者**:Huang J, et al.

**摘要**:本研究利用EHMT2特异性抗体(Western blot和免疫组化)分析了多种癌症细胞系及组织中EHMT2的蛋白表达水平,发现其在肺癌和结直肠癌中显著高表达。通过抗体阻断实验结合基因敲除,证实EHMT2通过调控H3K9me2修饰促进肿瘤细胞增殖和转移。

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2. **文献名称**:*Role of G9a in Neuronal Differentiation and Epigenetic Regulation*

**作者**:Shi Y, et al.

**摘要**:通过免疫荧光染色(使用EHMT2抗体)及ChIP-seq技术,研究者发现EHMT2在小鼠神经干细胞分化过程中介导H3K9二甲基化,沉默神经元抑制基因。抗体特异性验证实验显示,EHMT2缺失导致分化相关基因异常激活,提示其在神经发育中的关键作用。

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3. **文献名称**:*Pharmacological Inhibition of G9a/GLP Enhances Somatic Cell Reprogramming*

**作者**:Chen X, et al.

**摘要**:该研究使用EHMT2抗体检测组蛋白修饰变化,发现G9a/GLP抑制剂通过降低H3K9me2水平提高诱导多能干细胞(iPSC)生成效率。抗体结合流式细胞术证实,抑制EHMT2可解除体细胞重编程的表观遗传屏障。

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**备注**:以上文献均为示例,实际引用时需核实具体期刊名称及发表年份。如需全文检索,建议通过PubMed或Google Scholar输入关键词“EHMT2 antibody”、“G9a ChIP”等获取最新研究。

       

背景信息

The EHMT2 antibody targets the enzyme Euchromatic Histone Lysine Methyltransferase 2 (EHMT2. also known as G9a), a key regulator of epigenetic modifications. EHMT2 catalyzes the mono- and dimethylation of histone H3 at lysine 9 (H3K9me1/me2), a modification associated with transcriptional repression and heterochromatin formation. It plays critical roles in gene silencing, X-chromosome inactivation, and maintaining genomic stability through interactions with chromatin-remodeling complexes. Dysregulation of EHMT2 is linked to developmental disorders, cancer progression, and neurological diseases, making it a focus of therapeutic research.

EHMT2 antibodies are essential tools for studying its expression, localization, and function in cellular processes. They are widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate EHMT2's role in epigenetic regulation. Researchers also employ these antibodies to explore its involvement in diseases, such as its overexpression in certain cancers (e.g., leukemia, lung cancer) or its impact on neurodevelopmental pathways. Additionally, EHMT2 inhibitors are being evaluated for their potential to reverse aberrant methylation patterns in disease models. The antibody's specificity and validation are crucial for ensuring accurate interpretation of EHMT2-related mechanisms in both basic and clinical research contexts.

       
关键字: EHMT2抗体;EHMT2;EHMT2 Antibody;

公司简介

是一家科研、开发、生产和销售为一体的科技型企业,
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,蛋白组学,细胞生物学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:重组蛋白、抗体、生物试剂
  • 公司地址:青浦区
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