HTRA2抗体,Mouse Monoclonal HTRA2 Antibody
  • HTRA2抗体,Mouse Monoclonal HTRA2 Antibody
  • HTRA2抗体,Mouse Monoclonal HTRA2 Antibody
  • HTRA2抗体,Mouse Monoclonal HTRA2 Antibody

HTRA2抗体—艾普蒂

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-05-15
QQ交谈 微信洽谈

产品详情

中文名称:HTRA2抗体英文名称:Mouse Monoclonal HTRA2 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 1218 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: HTRA2
2025-05-15 HTRA2抗体 Mouse Monoclonal HTRA2 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 1218 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesOMI; MGCA8; PARK13; PRSS25
Entrez GeneID27429
clone6H7A8
WB Predicted band size48.8kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenPurified recombinant fragment of human HTRA2 (AA: 278-458) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Western blot analysis using HTRA2 mouse mAb against HL-60 (1), HepG2 (2), MCF-7 (3), Hela (4), PC-12 (5), C2C12 (6), and Raji (7) cell lysate.    


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       Flow cytometric analysis of Hela cells using HTRA2 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using HTRA2 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using HTRA2 mouse mAb with DAB staining.    


           

参考文献

以下是关于HTRA2抗体的3篇参考文献,涵盖其功能、结构及疾病相关研究:

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1. **文献名称**:*HtrA2 deficiency is associated with neurodegeneration and impaired mitochondrial proteolysis*

**作者**:Vande Walle, L. et al.

**摘要**:该研究探讨HTRA2(HtrA2/Omi)在帕金森病中的功能,利用基因敲除模型和HTRA2抗体检测发现,其缺失导致线粒体蛋白降解异常及多巴胺能神经元退化,提示其通过调控线粒体稳态参与神经退行机制。

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2. **文献名称**:*Crystal structure of HtrA2 reveals a conformational switch for protease activation*

**作者**:Clausen, T. et al.

**摘要**:通过X射线晶体学解析HTRA2的三维结构,结合抗体标记技术阐明其活性调控机制,发现其PDZ结构域介导的构象变化对蛋白酶活性至关重要,为靶向药物设计提供结构基础。

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3. **文献名称**:*Mitochondrial release of HtrA2 promotes apoptosis during cellular stress*

**作者**:Suzuki, Y. et al.

**摘要**:研究显示,在凋亡刺激下,HTRA2从线粒体释放至胞质,通过HTRA2抗体追踪其定位变化,证实其通过切割凋亡抑制蛋白(如XIAP)促进caspase激活,揭示其在应激诱导凋亡中的关键作用。

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如需具体文献DOI或补充更多研究,可进一步缩小范围或指定研究领域。

       

背景信息

**Background of HTRA2 Antibody**

HTRA2 (High-temperature requirement A2), also known as OMI, is a mitochondrial serine protease belonging to the evolutionarily conserved HtrA protein family. Primarily localized in the mitochondrial intermembrane space, HTRA2 plays dual roles in cellular homeostasis: it maintains mitochondrial protein quality under stress by degrading misfolded proteins and participates in apoptosis by cleaving inhibitor of apoptosis proteins (IAPs) upon its release into the cytosol. Dysregulation of HTRA2 is linked to neurodegenerative disorders, particularly Parkinson’s disease, where loss-of-function mutations (e.g., in the *PARK13* gene) impair protease activity, leading to mitochondrial dysfunction and neuronal death.

HTRA2 antibodies are essential tools for studying its expression, localization, and function. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding research into HTRA2’s role in apoptosis, mitochondrial dynamics, and disease mechanisms. These antibodies also help explore therapeutic strategies, such as targeting HTRA2 in cancer (where its pro-apoptotic activity may suppress tumors) or mitigating its dysfunction in neurodegeneration. By facilitating precise protein analysis, HTRA2 antibodies contribute to understanding cellular stress responses and developing targeted therapies.

       
关键字: HTRA2抗体;HTRA2;HTRA2 Antibody;

公司简介

是一家科研、开发、生产和销售为一体的科技型企业,
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,蛋白组学,细胞生物学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
普通会员
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:重组蛋白、抗体、生物试剂
  • 公司地址:青浦区
询盘

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