H2AFX抗体,Mouse Monoclonal H2AFX Antibody
  • H2AFX抗体,Mouse Monoclonal H2AFX Antibody
  • H2AFX抗体,Mouse Monoclonal H2AFX Antibody
  • H2AFX抗体,Mouse Monoclonal H2AFX Antibody

H2AFX抗体—艾普蒂

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-05-16
QQ交谈 微信洽谈

产品详情

中文名称:H2AFX抗体英文名称:Mouse Monoclonal H2AFX Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 1522 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: H2AFX
2025-05-16 H2AFX抗体 Mouse Monoclonal H2AFX Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 1522 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/50 - 1/200 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

AliasesH2AX; H2A.X; H2A/X
Entrez GeneID3014
clone4A2D5
WB Predicted band size15.1kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human H2AFX expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

+ +

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       Western blot analysis using H2AFX mouse mAb against HEK293 (1), HepG2 (2), Jurkat (3),Hela (4), Raji (5), K562 (6) and HCT116 (7) cell lysate.    


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       Immunofluorescence analysis of Hela cells using H2AFX mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of Hela cells using H2AFX mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using H2AFX mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using H2AFX mouse mAb with DAB staining.    


           

参考文献

以下是3篇关于H2AFX(或γ-H2AX)抗体的经典文献及其摘要内容:

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1. **文献名称**:*DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139*

**作者**:Rogakou, E.P., Pilch, D.R., Orr, A.H., Ivanova, V.S., Bonner, W.M.

**摘要**:首次报道了H2AFX在DNA双链断裂(DSBs)后被磷酸化形成γ-H2AX的过程,并开发了特异性抗体用于检测。该研究揭示了γ-H2AX作为DSBs的早期分子标记,为后续DNA损伤修复研究奠定基础。

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2. **文献名称**:*Evidence for a lack of DNA double-strand break repair in human cells exposed to very low x-ray doses*

**作者**:Rothkamm, K., Löbrich, M.

**摘要**:利用γ-H2AX抗体通过免疫荧光技术,证明即使低剂量辐射(低至1 mGy)也会在人类细胞中诱导可检测的DSBs。研究表明γ-H2AX抗体可作为高灵敏工具评估DNA损伤程度。

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3. **文献名称**:*γH2AX as a biomarker of DNA damage induced by ionizing radiation in human peripheral blood lymphocytes and tumor cell lines*

**作者**:Sedelnikova, O.A., Rogakou, E.P., Panyutin, I.G., Bonner, W.M.

**摘要**:系统评估了γ-H2AX抗体在定量检测电离辐射引起的DNA损伤中的应用,验证了其在淋巴细胞和肿瘤细胞中的可靠性,提出其作为临床辐射暴露生物标志物的潜力。

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如需具体实验步骤或应用案例,可进一步补充相关文献。

       

背景信息

The H2AFX antibody is a crucial tool in studying the DNA damage response and genomic stability. H2AFX, a variant of histone H2A, is rapidly phosphorylated at serine 139 (forming γH2AX) near DNA double-strand breaks (DSBs), a process mediated by kinases like ATM, ATR, or DNA-PK. This phosphorylation marks sites of DNA damage, recruiting repair proteins and facilitating chromatin remodeling. The γH2AX foci serve as biomarkers for DSBs, making H2AFX antibodies essential in detecting and quantifying DNA damage in cells.

Researchers use H2AFX antibodies in techniques such as immunofluorescence, Western blotting, and flow cytometry to assess genomic instability in cancer, aging, and radiation biology. These antibodies help evaluate the efficacy of DNA-damaging therapies (e.g., chemotherapy, radiotherapy) and study mechanisms of DNA repair pathways. Specificity for the phosphorylated form (γH2AX) is critical, as it distinguishes active damage signaling from baseline H2AFX. Commercial H2AFX antibodies are typically validated across human, mouse, and rat samples, though species reactivity should be confirmed. Proper controls, like irradiated cells (positive) or phosphatase-treated samples (negative), ensure accurate interpretation. Understanding H2AFX dynamics via these antibodies advances insights into diseases linked to genomic instability and therapeutic strategies targeting DNA repair.

       
关键字: H2AFX抗体;H2AFX;H2AFX Antibody;

公司简介

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成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,蛋白组学,细胞生物学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:重组蛋白、抗体、生物试剂
  • 公司地址:青浦区
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