TP53BP1抗体—艾普蒂

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Flow cytometric analysis of HepG2 cells using TP53BP1 mouse mAb (green) and negative control (purple).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using TP53BP1 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using TP53BP1 mouse mAb with DAB staining.    

  
  

The TP53BP1 (Tumor Protein p53 Binding Protein 1) antibody is a critical tool for studying the role of the TP53BP1 protein in DNA damage response (DDR) and genome stability. TP53BP1. also known as 53BP1. is a key mediator protein involved in double-strand break (DSB) repair, particularly promoting non-homologous end joining (NHEJ) over homologous recombination (HR). It localizes to DNA lesions through interactions with histone H2A ubiquitination (H2AK15ub) and methylated histone H4 (H4K20me2), facilitating chromatin recruitment of downstream repair factors. TP53BP1 also regulates cell cycle checkpoints, apoptosis, and immunoglobulin class switching in B cells.

Antibodies targeting TP53BP1 are widely used in research to detect its expression, phosphorylation status (e.g., at Ser1778), and subcellular localization via techniques like Western blotting, immunofluorescence, and immunohistochemistry. These antibodies help elucidate TP53BP1's role in cancer biology, as its dysfunction is linked to genomic instability, tumor progression, and resistance to therapies like PARP inhibitors. Additionally, TP53BP1 antibodies are employed in studying crosstalk between DDR pathways, replication stress, and mechanisms underlying diseases such as immunodeficiency or developmental disorders.

Validated for specificity across human, mouse, and other model systems, TP53BP1 antibodies (e.g., clones like 19/Human 53BP1) are indispensable for exploring DNA repair dynamics, biomarker discovery, and therapeutic targeting in oncology and molecular pathology research.