**Background of ATAD3A Antibody**
ATAD3A (ATPase family AAA domain-containing protein 3A) is a mitochondrial membrane-anchored protein critical for maintaining mitochondrial structure, function, and communication with the endoplasmic reticulum. It belongs to the AAA+ ATPase family, characterized by conserved ATP-binding and hydrolysis domains. ATAD3A plays roles in mitochondrial DNA (mtDNA) organization, cholesterol metabolism, and regulation of apoptosis and autophagy. Dysregulation of ATAD3A is linked to neurodegenerative disorders, cardiomyopathies, and cancers, with overexpression observed in tumors like glioblastoma and hepatocellular carcinoma.
ATAD3A antibodies are essential tools for studying its expression, localization, and interactions. They are widely used in techniques such as Western blot (WB), immunofluorescence (IF), and immunohistochemistry (IHC) to investigate ATAD3A’s role in mitochondrial dynamics and disease mechanisms. Recent studies highlight its involvement in mitochondrial protein synthesis and its interaction with proteins like SAMM50 and PHB2. Notably, ATAD3A mutations are associated with severe neurodevelopmental disorders, underscoring its importance in cellular health. Antibodies targeting specific epitopes (e.g., N-terminal or C-terminal regions) help dissect its multifunctional domains and post-translational modifications, aiding both basic research and clinical diagnostics.