**Background of DENR Antibody**
The DENR (Density-Regulated Protein) antibody is a tool used to detect and study the DENR protein, a conserved eukaryotic factor involved in translation regulation. DENR, also known as density-regulated re-initiation and release factor, interacts with MCT-1 (malignant T-cell-amplified sequence 1) to form a complex critical for ribosome recycling and translation re-initiation, particularly for mRNAs with short upstream open reading frames (uORFs). This process influences cellular responses to stress, proliferation, and differentiation.
DENR's role in translation has linked it to cancer biology, as dysregulation of DENR-MCT-1 is observed in malignancies, impacting oncogene expression and tumor progression. Antibodies targeting DENR enable researchers to investigate its expression, localization, and function in cells or tissues. They are used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to explore DENR's involvement in diseases or developmental processes.
Research using DENR antibodies has also shed light on its evolutionary conservation and structural features, revealing similarities to translation initiation factors. These studies underscore DENR's importance in fundamental biological mechanisms and its potential as a therapeutic target in cancer and other pathologies driven by aberrant translation control.