The UBE2D3 antibody is a tool used to detect and study the ubiquitin-conjugating enzyme E2 D3 (UBE2D3), a key component of the ubiquitin-proteasome system. UBE2D3. also known as UBCH5C, belongs to the E2 enzyme family responsible for transferring ubiquitin molecules to substrate proteins, typically in collaboration with E3 ubiquitin ligases. This process tags target proteins for degradation, regulates cellular processes like DNA repair, cell cycle progression, and signal transduction, and is implicated in diseases including cancer and neurodegenerative disorders.
UBE2D3 antibodies are widely used in research to investigate protein ubiquitination dynamics, enzyme-substrate interactions, and disease mechanisms. They enable techniques such as Western blotting, immunoprecipitation, and immunofluorescence to assess UBE2D3 expression levels, localization, and activity across tissues. Studies have shown UBE2D3's role in modulating pathways like NF-κB signaling and its overexpression in certain cancers, making it a potential therapeutic target.
These antibodies vary in specificity, with some recognizing conserved regions across species (human, mouse, rat) or distinguishing UBE2D3 from closely related family members (UBE2D1. D2. D4). Validation often includes knockout controls to confirm minimal cross-reactivity. Researchers rely on UBE2D3 antibodies to explore its dual roles in both promoting and inhibiting tumorigenesis, highlighting its context-dependent functions in cellular homeostasis and disease.