The RNF2 (Ring Finger Protein 2) antibody is a critical tool for studying the role of RNF2. a key component of the Polycomb Repressive Complex 1 (PRC1), which regulates gene silencing and epigenetic modifications. RNF2. also known as RING1B, functions as an E3 ubiquitin ligase, catalyzing the monoubiquitination of histone H2A at lysine 119 (H2AK119ub). This modification is essential for maintaining transcriptional repression of developmental genes, influencing processes like cell differentiation, proliferation, and stem cell pluripotency. Dysregulation of RNF2 has been linked to cancer progression, developmental disorders, and other diseases.
RNF2 antibodies are widely used in techniques such as Western blotting, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunofluorescence to detect protein expression, localization, and interaction partners. These antibodies help researchers investigate RNF2’s involvement in chromatin remodeling, its partnership with other Polycomb group proteins, and its impact on cellular pathways. High-quality RNF2 antibodies are validated for specificity, often through knockout/knockdown controls, and are available in various host species (e.g., rabbit, mouse) with different conjugates (e.g., HRP, fluorescent tags). Their applications span cancer research, stem cell biology, and developmental studies, offering insights into therapeutic targeting of epigenetic mechanisms.