The Id1 (Inhibitor of DNA Binding 1) antibody is a crucial tool for studying the Id1 protein, a member of the helix-loop-helix (HLH) transcription factor family. Id proteins lack a DNA-binding domain and function as dominant-negative regulators of basic HLH (bHLH) transcription factors by forming non-functional heterodimers with them. Id1 is implicated in diverse cellular processes, including cell cycle progression, differentiation, angiogenesis, and apoptosis. Overexpression of Id1 has been linked to tumorigenesis, metastasis, and poor prognosis in cancers such as breast, prostate, and glioblastoma, due to its role in promoting cell proliferation and epithelial-mesenchymal transition (EMT).
Id1 antibodies are widely used in research to detect and quantify Id1 expression in tissues or cells via techniques like Western blotting, immunohistochemistry (IHC), and flow cytometry. They are essential for exploring Id1's involvement in developmental biology, stem cell maintenance, and cancer progression. Commercially available Id1 antibodies are typically raised in rabbits or mice, targeting specific epitopes within the Id1 protein. Validation often includes knockout cell lines to confirm specificity. Recent studies focus on Id1's role in tumor microenvironments, therapy resistance, and as a potential therapeutic target. Reliable Id1 antibodies remain vital for advancing our understanding of its regulatory mechanisms in health and disease.