The TRAM2 (Translocation-Associated Membrane Protein 2) antibody is a tool used to study the TRAM2 protein, a component of the endoplasmic reticulum (ER) membrane involved in protein translocation and post-translational modifications. TRAM2 facilitates the secretion and proper folding of collagen by interacting with enzymes like prolyl 4-hydroxylase, critical for collagen hydroxylation and stability. Dysregulation of TRAM2 has been linked to osteogenesis imperfecta, metabolic disorders, and cancer progression due to its role in collagen biosynthesis and ER stress responses.
TRAM2 antibodies, often raised in rabbits or mice, enable the detection and analysis of TRAM2 expression in techniques such as Western blotting, immunofluorescence, and immunohistochemistry. These antibodies help researchers investigate TRAM2's interaction with translocon complexes (e.g., SEC61) and its regulatory effects on lipid metabolism, particularly in adipocytes. Studies also utilize TRAM2 antibodies to explore its tissue-specific expression patterns, with higher levels observed in collagen-rich tissues like bone, skin, and liver.
As a research tool, TRAM2 antibodies contribute to understanding ER-associated processes, collagen-related pathologies, and potential therapeutic targets for connective tissue disorders. Their specificity and validation across species (human, mouse, rat) make them valuable for mechanistic studies in cell biology and disease models.