KLHL8 (Kelch-like family member 8) is a member of the Kelch-like protein family, characterized by its conserved BTB (Broad-Complex, Tramtrack, and Bric-à-brac) domain, BACK (BTB and C-terminal Kelch) domain, and Kelch repeat motifs. These structural features enable KLHL8 to function as a substrate-specific adaptor for Cullin3 (CUL3)-based E3 ubiquitin ligase complexes, facilitating the ubiquitination and subsequent proteasomal degradation of target proteins. KLHL8 is implicated in regulating cellular processes such as cytoskeletal organization, signal transduction, and protein quality control. Dysregulation of KLHL8 has been linked to various pathologies, including cancer, neurodegenerative disorders, and cardiovascular diseases, underscoring its potential role in disease mechanisms.
Antibodies targeting KLHL8 are critical tools for investigating its expression, localization, and functional interactions. These antibodies are commonly used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to study KLHL8's protein levels, binding partners, and tissue distribution. Given the structural homology among Kelch-like proteins, specificity validation (e.g., via knockout controls) is essential to ensure antibody reliability. Research utilizing KLHL8 antibodies has shed light on its involvement in ubiquitin-dependent pathways and disease contexts, making it a focus for therapeutic targeting and biomarker discovery. Current studies aim to clarify its substrate profiles and regulatory mechanisms in both physiological and pathological states.