The TBX5 antibody is a crucial tool in studying the TBX5 protein, a member of the T-box transcription factor family essential for embryonic development, particularly in heart and limb formation. TBX5 plays a pivotal role in regulating gene expression during organogenesis, with mutations linked to congenital disorders like Holt-Oram syndrome, characterized by heart defects and upper limb abnormalities. The antibody is widely used in research to detect TBX5 expression in tissues, investigate its functional mechanisms, and explore its interactions with other developmental regulators (e.g., GATA4. NKX2-5).
Developed using immunogenic peptides or recombinant proteins, TBX5 antibodies are validated for specificity in assays such as Western blot, immunohistochemistry, and immunofluorescence. They enable visualization of TBX5 localization in nuclei, confirming its role as a DNA-binding transcription factor. Commercial availability from biotech vendors has facilitated studies on TBX5's involvement in cardiac chamber specification, conduction system development, and disease modeling. Recent applications extend to stem cell differentiation and regenerative medicine, where TBX5 guides in vitro cardiomyocyte programming.
Despite its utility, researchers must verify antibody performance across species and experimental conditions due to potential cross-reactivity with related T-box proteins. Proper controls ensure accurate interpretation of TBX5's spatiotemporal expression patterns in developmental and pathological contexts.