CLIC6 (Chloride Intracellular Channel 6) is a member of the CLIC protein family, which exhibits dual functionality as both soluble and membrane-associated proteins involved in chloride ion transport and cellular homeostasis. CLIC6 is characterized by a conserved glutathione S-transferase (GST)-like domain, enabling its participation in redox-sensitive processes. Unlike other CLIC members, CLIC6 shows restricted tissue expression, with higher levels observed in the brain, kidney, and reproductive organs, suggesting organ-specific roles in ion regulation, vesicular trafficking, or organelle maturation. Its precise physiological mechanisms remain under investigation, but studies link CLIC6 to endosomal-lysosomal pathways, autophagy, and potential roles in neurodegenerative diseases or cancer progression.
CLIC6 antibodies are essential tools for detecting and characterizing the protein’s expression, localization, and function in research. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study CLIC6’s subcellular distribution, particularly in membrane-bound compartments or vesicles. Commercially available CLIC6 antibodies are typically raised against specific peptide epitopes, and their validation includes checks for cross-reactivity with other CLIC family members. Research utilizing these antibodies has explored CLIC6’s involvement in pathological conditions, such as its dysregulation in tumors or association with lysosomal storage disorders. However, challenges persist in understanding its exact channel activation mechanisms and interaction partners, highlighting the need for further antibody-based studies to clarify its biological and clinical significance.