HIPK2抗体,Rabbit Polyclonal HIPK2 Antibody
  • HIPK2抗体,Rabbit Polyclonal HIPK2 Antibody
  • HIPK2抗体,Rabbit Polyclonal HIPK2 Antibody
  • HIPK2抗体,Rabbit Polyclonal HIPK2 Antibody

HIPK2抗体;HIPK2 Antibody—艾普蒂

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-05-28
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产品详情

中文名称:HIPK2抗体英文名称:Rabbit Polyclonal HIPK2 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 7110 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: HIPK2
2025-05-28 HIPK2抗体 Rabbit Polyclonal HIPK2 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 7110 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/30-1/150 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/5000-1/10000 Human,Mouse,Rat
   

产品详情

AliasesPRO0593
WB Predicted band size131 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenSynthetic peptide of human HIPK2
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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       Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: 231 cell lysate, Primary antibody: P13173(HIPK2 Antibody) at dilution 1/850, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes    


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       The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P13173(HIPK2 Antibody) at dilution 1/40. (Original magnification: ×200)    


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       The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P13173(HIPK2 Antibody) at dilution 1/40. (Original magnification: ×200)    


           

参考文献

以下是关于HIPK2抗体的3篇参考文献示例(注:文献信息为示例性质,实际引用时请核实来源):

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1. **文献名称**: "HIPK2 modulates p53 activity through direct phosphorylation"

**作者**: D’Orazi G., et al.

**摘要**: 本研究利用特异性HIPK2抗体,通过免疫共沉淀和Western blot技术,揭示了HIPK2在DNA损伤条件下通过磷酸化p53蛋白(Ser46位点)增强其促凋亡功能的机制,证实了HIPK2在细胞应激反应中的关键作用。

2. **文献名称**: "Regulation of HIPK2 subcellular localization and kinase activity by SUMOylation"

**作者**: Rinaldo C., et al.

**摘要**: 作者通过免疫荧光和HIPK2抗体分析,发现SUMO修饰调控HIPK2的核质转运及其激酶活性,进而影响其对转录因子(如CTBP)的磷酸化能力,揭示了HIPK2在肿瘤发生中的潜在调控途径。

3. **文献名称**: "HIPK2 deficiency attenuates TGF-β-induced fibrosis via suppression of SMAD signaling"

**作者**: Shen H., et al.

**摘要**: 研究使用HIPK2敲除小鼠模型及特异性抗体检测,证明HIPK2通过增强SMAD蛋白磷酸化促进TGF-β信号传导,在组织纤维化中起关键作用,为靶向HIPK2的抗纤维化治疗提供理论依据。

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**注意**:以上文献为示例,实际引用时需根据具体研究内容查阅真实发表的论文(建议通过PubMed或Google Scholar搜索关键词“HIPK2 antibody”或“HIPK2 kinase function”获取准确信息)。

       

背景信息

**Background of HIPK2 Antibodies**

HIPK2 (Homeodomain-Interacting Protein Kinase 2) is a serine/threonine kinase involved in diverse cellular processes, including stress signaling, DNA damage response, apoptosis, and proliferation. It regulates transcription factors (e.g., p53. Wnt/β-catenin) and signaling pathways (e.g., TGF-β, JNK), linking it to diseases like cancer, neurodegeneration, and fibrosis. HIPK2 activity is modulated by post-translational modifications (phosphorylation, ubiquitination) and subcellular localization, often accumulating in nuclear speckles.

HIPK2 antibodies are essential tools for studying its expression, localization, and function. They detect endogenous HIPK2 in techniques like Western blotting, immunofluorescence, and immunoprecipitation. Due to HIPK2’s multiple isoforms and homology with HIPK1/3. antibody specificity is critical. Some antibodies target unique regions (e.g., kinase domain, C-terminal), while others distinguish phosphorylated forms (e.g., at Thr-880/Ser-882). Validation using knockout cells or siRNA is recommended to confirm selectivity.

Challenges include cross-reactivity, variable isoform detection, and sensitivity to HIPK2’s low abundance or degradation. Researchers use these antibodies to explore HIPK2’s role in diseases, such as its tumor-suppressive or oncogenic duality in cancer, or its regulation of fibrosis pathways. Reliable HIPK2 antibodies advance mechanistic insights and therapeutic targeting in biomedical research.

       
关键字: HIPK2抗体;HIPK2;HIPK2 Antibody;

公司简介

生物技术有限公司
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,植物生物技术,细胞培养,蛋白组学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:生物试剂
  • 公司地址:青浦
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