PKM2抗体,Rabbit Monoclonal PKM2 Antibody
  • PKM2抗体,Rabbit Monoclonal PKM2 Antibody
  • PKM2抗体,Rabbit Monoclonal PKM2 Antibody
  • PKM2抗体,Rabbit Monoclonal PKM2 Antibody

PKM2抗体;PKM2 Antibody—艾普蒂

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-05-29
QQ交谈 微信洽谈

产品详情

中文名称:PKM2抗体英文名称:Rabbit Monoclonal PKM2 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 7944 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: PKM2
2025-05-29 PKM2抗体 Rabbit Monoclonal PKM2 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 7944 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB1/1000-1/2000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHCIHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC1/50-1/200 Human,Mouse,Rat
FCM1/20-1/100 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

AliasesCTHBP; Cytosolic thyroid hormone binding protein; KPYM; OIP 3; Oip3; OIP3; OPA interacting protein 3; p58; PK Muscle type; muscle type; PK2; Pk3; PKM2;PKM
WB Predicted band size58 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human PKM
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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       Western blot analysis of PKM2 expression in HeLa cell lysate.    


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       All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    


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       All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    


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       Immunohistochemical analysis of paraffin-embedded human cervix cancer, using PKM2 Antibody.    


           

参考文献

以下是3篇关于PKM2抗体的经典文献摘要概括:

1. **文献名称**: *PKM2 Isoform-Specific Deletion Reveals a Differential Requirement for Pyruvate Kinase in Tumor Cells*

**作者**: Israelsen, W.J., et al.

**摘要**: 该研究利用PKM2特异性抗体验证基因敲除模型,证明PKM2在肿瘤细胞代谢重编程中的关键作用,尤其在Warburg效应和细胞增殖中不可或缺。

2. **文献名称**: *Tyrosine Phosphorylation Inhibits PKM2 to Promote the Warburg Effect and Tumor Growth*

**作者**: Hitosugi, T., et al.

**摘要**: 通过PKM2抗体检测磷酸化修饰,揭示酪氨酸磷酸化抑制PKM2酶活性,促进肿瘤细胞糖酵解和生长,为靶向PKM2的癌症治疗提供依据。

3. **文献名称**: *PKM2 Regulates the Warburg Effect and Promotes Tumorigenesis*

**作者**: Christofk, H.R., et al.

**摘要**: 研究使用PKM2特异性抗体证实其表达与肿瘤发生相关,PKM2通过调控糖酵解通量促进肿瘤细胞增殖,并影响基因表达和氧化应激反应。

这些文献均通过PKM2抗体进行蛋白检测或功能研究,聚焦其在肿瘤代谢中的调控机制。

       

背景信息

PKM2 (pyruvate kinase M2) is a key isoform of the pyruvate kinase enzyme family, which catalyzes the final step of glycolysis by converting phosphoenolpyruvate to pyruvate while generating ATP. Unlike other isoforms, PKM2 is preferentially expressed in rapidly proliferating cells, including cancer cells, where it plays a dual role in regulating metabolic reprogramming and gene transcription. Its unique ability to switch between a highly active tetrameric form (promoting oxidative metabolism) and a less active dimeric form (redirecting glycolytic intermediates to biosynthetic pathways) underpins the Warburg effect, a hallmark of cancer metabolism. PKM2 is also involved in non-metabolic functions, such as acting as a protein kinase or transcriptional coactivator, influencing cell cycle progression, apoptosis, and tumorigenesis.

PKM2 antibodies are essential tools for studying its expression, localization, and post-translational modifications in both normal and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate PKM2's role in cancer progression, metabolic disorders, and therapeutic targeting. Elevated PKM2 levels are correlated with poor prognosis in various cancers, making it a potential biomarker and therapeutic target. Researchers also utilize these antibodies to explore PKM2's interaction with oncogenic signaling pathways (e.g., HIF-1α, mTOR) and its regulation by metabolites or phosphorylation. Developing specific PKM2 antibodies remains critical for dissecting its complex roles in health and disease.

       
关键字: PKM2抗体;PKM2;PKM2 Antibody;

公司简介

生物技术有限公司
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,植物生物技术,细胞培养,蛋白组学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:生物试剂
  • 公司地址:青浦
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