ACSS2抗体;ACSS2 Antibody—艾普蒂

+ +

•  

   

     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

•  

   

     Western blot analysis of ACSS2 expression in HepG2 cell lysate.    

  
  

•  

   

     Immunofluorescent analysis using the Antibody at 1:50 dilution.    

  
  

The ACSS2 (acyl-CoA synthetase short-chain family member 2) antibody is a crucial tool for studying the ACSS2 enzyme, which plays a pivotal role in cellular metabolism. ACSS2 catalyzes the conversion of acetate into acetyl-CoA, a key substrate for energy production, lipid synthesis, and protein acetylation. This enzyme is particularly significant in low-glucose or high-acetate conditions, where it helps maintain cellular acetyl-CoA levels, influencing processes like histone acetylation and gene regulation. Dysregulation of ACSS2 has been implicated in cancer, metabolic disorders, and neurodegenerative diseases, making it a target for therapeutic research.

ACSS2 antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect ACSS2 expression, localization, and abundance in tissues or cell lines. Specificity is validated through knockdown/knockout controls or blocking peptide assays. Researchers employ these antibodies to explore ACSS2's role in tumor progression (e.g., supplying acetyl-CoA for cancer cell growth), metabolic adaptation, and epigenetic regulation. Recent studies also highlight its involvement in cellular stress responses, making ACSS2 antibodies essential for dissecting metabolic pathways in health and disease.