EBP50抗体;EBP50 Antibody—艾普蒂 新品

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     Western blot analysis of EBP50 expression in (1) HepG2 cell lysate; (2) PC12 cell lysate.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

The EBP50 (Ezrin-Radixin-Moesin Binding Phosphoprotein 50) antibody is a key tool for studying the scaffolding protein EBP50. also known as Na+/H+ Exchanger Regulatory Factor 1 (NHERF1). EBP50. encoded by the SLC9A3R1 gene, plays a critical role in organizing membrane-associated protein complexes by linking transmembrane proteins to cytoskeletal components. It contains two PDZ domains that mediate interactions with various signaling molecules (e.g., β-catenin, PTEN, EGFR) and an ERM-binding domain that connects to actin via ezrin/radixin/moesin proteins. This protein is essential for regulating ion transport, receptor recycling, and signal transduction pathways involved in cell polarity, migration, and tumorigenesis.

EBP50's dual role in cancer—acting as either a tumor suppressor or promoter depending on cellular context—has driven significant research interest. Dysregulation of EBP50 is implicated in breast, prostate, and lung cancers, as well as neurological disorders like Alzheimer's disease. Antibodies targeting EBP50 are widely used in Western blotting, immunofluorescence, and immunohistochemistry to analyze its expression, subcellular localization, and post-translational modifications. Researchers prioritize antibodies validated for specific applications and isoforms, as EBP50 exists in multiple phosphorylated states and splice variants with distinct functions. These reagents are indispensable for deciphering EBP50's complex roles in health and disease.