The mSin3A antibody is a crucial tool in studying the mammalian Sin3A protein, a key component of the Sin3/HDAC (histone deacetylase) corepressor complex. mSin3A (mammalian Sin3A) functions as a scaffold protein, recruiting histone deacetylases (e.g., HDAC1/2) and other chromatin-modifying enzymes to regulate gene expression through chromatin remodeling and transcriptional repression. It plays essential roles in diverse cellular processes, including cell cycle progression, differentiation, and apoptosis, and is implicated in cancer, neurological disorders, and metabolic diseases.
The mSin3A antibody specifically detects endogenous mSin3A isoforms (∼150 kDa) in various experimental applications, such as Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunofluorescence (IF). Developed in hosts like rabbit or mouse, these antibodies are often validated for specificity using knockout cell lines or siRNA-mediated knockdown. Researchers rely on mSin3A antibodies to investigate its interactions with transcription factors (e.g., Mad, p53), its role in epigenetic silencing, and its dysregulation in disease contexts. Commercial variants may differ in epitope recognition (e.g., N-terminal vs. C-terminal regions) or conjugation (e.g., HRP, fluorescent tags), allowing flexibility in experimental design. Proper controls, such as peptide blocking or comparative analysis with Sin3B-specific antibodies, are critical to ensure accurate data interpretation.