NCS1抗体;NCS1 Antibody—艾普蒂

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     Western blot analysis of NCS1 expression in 293T cell lysate.    

  
  

The Neuronal Calcium Sensor 1 (NCS1) protein, also known as frequenin, is a calcium-binding protein belonging to the EF-hand calcium sensor family. It plays a critical role in regulating calcium-dependent signaling pathways, particularly in neuronal and neuroendocrine cells. NCS1 is involved in diverse cellular processes, including neurotransmitter release, synaptic plasticity, and ion channel modulation, by interacting with targets like G-protein-coupled receptors (GPCRs), phosphatidylinositol 4-kinase, and voltage-gated calcium channels. Dysregulation of NCS1 expression or function has been implicated in neurological and psychiatric disorders, such as schizophrenia, bipolar disorder, and Parkinson’s disease, as well as cancer progression.

NCS1 antibodies are essential tools for studying the localization, expression, and molecular interactions of NCS1 in both physiological and pathological contexts. These antibodies are generated using immunogenic peptides or recombinant NCS1 protein, enabling detection through techniques like Western blotting, immunohistochemistry, and immunoprecipitation. Specificity and validation are critical, as cross-reactivity with other EF-hand proteins (e.g., recoverin, neurocalcin) must be ruled out. Research using NCS1 antibodies has advanced understanding of its role in calcium homeostasis, synaptic transmission, and disease mechanisms. For example, studies reveal altered NCS1 levels in postmortem brain tissues of psychiatric patients, highlighting its potential as a biomarker or therapeutic target. Ongoing work focuses on elucidating its interactions with dopamine receptors and implications in drug addiction mechanisms.