Phospho-Syk (Tyr323) antibody is a specialized tool used to detect the activated form of spleen tyrosine kinase (Syk), a non-receptor tyrosine kinase critical in immune receptor signaling. Syk plays a central role in transmitting signals from B-cell receptors (BCR), Fc receptors, and other immunoreceptors. Upon receptor engagement, Syk undergoes phosphorylation at specific tyrosine residues, including Tyr323. which is located in its linker region. This phosphorylation event is essential for Syk’s activation, enabling downstream signaling pathways like MAPK, PI3K/AKT, and NF-κB, which regulate immune cell activation, proliferation, and survival.
The Phospho-Syk (Tyr323) antibody specifically recognizes Syk when phosphorylated at Tyr323. making it invaluable for studying Syk activation dynamics in immune responses, inflammation, and oncogenic signaling. Researchers use this antibody in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate Syk’s role in diseases like B-cell malignancies, autoimmune disorders (e.g., rheumatoid arthritis), and allergic reactions. Its specificity is often validated using cell lines with Syk knockout or phosphorylation site mutations to ensure minimal cross-reactivity.
Understanding Syk phosphorylation at Tyr323 also has therapeutic implications, as Syk inhibitors are being explored for treating autoimmune and inflammatory conditions. This antibody aids in evaluating drug efficacy and mechanistic studies, providing insights into Syk-driven pathological processes.