Phospho-CaMKII (Thr305)抗体;Phospho-CaMKII (Thr305) Antibody—艾普蒂 新品

The Phospho-CaMKII (Thr305) antibody detects the phosphorylated form of calcium/calmodulin-dependent protein kinase II (CaMKII) at threonine 305. a key regulatory site in this multifunctional serine/threonine kinase. CaMKII plays a central role in calcium signaling pathways, particularly in neurons and cardiac cells, where it regulates processes such as synaptic plasticity, memory formation, and excitation-contraction coupling. Autophosphorylation at Thr286 (in the α-isoform) is a well-characterized activation mechanism, conferring calcium-independent activity. In contrast, phosphorylation at Thr305 (and the adjacent Thr306 in some isoforms) occurs within the autoinhibitory domain and is associated with reduced CaMKII activity. This modification is mediated by other kinases, such as PKA, and may serve as a feedback mechanism to limit excessive CaMKII activation or redirect its substrate specificity. The Phospho-CaMKII (Thr305) antibody is widely used in neuroscience and cardiovascular research to study CaMKII regulation under physiological and pathological conditions, including neurological disorders, cardiac hypertrophy, and arrhythmias. Its specificity for the phosphorylated state allows researchers to investigate dynamic post-translational modifications linked to cellular stress, signaling crosstalk, and disease mechanisms. Validation typically involves knockout controls or phosphatase treatment to confirm phosphorylation-dependent recognition.