beta Catenin抗体;beta Catenin Antibody—艾普蒂

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     Western blot analysis of beta Catenin (7E2)in Hela, 293T, mouse Liver, rat Liver lysates using βCatenin antibody.    

  
  

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     Western blot analysis of beta Catenin in T47D, 3T3, COS7 and C6 lysates using βCatenin antibody.    

  
  

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     Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using beta Catenin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    

  
  

Beta-catenin is a multifunctional protein that plays a pivotal role in both cell-cell adhesion and Wnt signaling pathways. As a key component of the cadherin complex, it contributes to structural integrity at adherens junctions. In the canonical Wnt pathway, beta-catenin acts as a transcriptional co-activator: when Wnt signals are absent, it is phosphorylated and targeted for proteasomal degradation via the "destruction complex" (APC, Axin, GSK3β). Wnt activation stabilizes beta-catenin, allowing its nuclear translocation to regulate genes involved in cell proliferation, differentiation, and embryonic development. Dysregulation of beta-catenin, often due to mutations in *CTNNB1* (its encoding gene) or upstream regulators (e.g., APC in colorectal cancer), is implicated in various cancers, fibrotic diseases, and developmental disorders.

Beta-catenin antibodies are essential tools for studying its localization, expression, and signaling activity. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess beta-catenin's cytoplasmic/nuclear accumulation—a hallmark of Wnt pathway activation. Specific antibodies target distinct epitopes (e.g., N-terminal phosphorylation sites, C-terminal transactivation domains) or post-translational modifications. Some antibodies differentiate between active (non-phosphorylated) and inactive forms. Validated antibodies are critical for diagnostic applications (e.g., identifying Wnt-driven tumors) and mechanistic research in cancer biology, stem cell regulation, and tissue homeostasis. Commercial antibodies are typically raised in rabbits or mice, with clones like E-5 and D10A8 being commonly referenced. Proper validation (knockout controls, functional assays) ensures specificity, as cross-reactivity with other catenins or proteins can yield misleading results.