Human IL-33 (C208S, C227S, C232S, C259S) Protein, His Tag (MALS verified)
分子别名（Synonym）
IL33, DV27, C9ORF26, IL1F11, NFHEV, DKFZp586H0523, DVS27, NFEHEV, RP11-575C20.2
表达区间及表达系统（Source）
Human IL-33 (C208S, C227S, C232S, C259S) Protein, His Tag (IL3-H52H6) is expressed from human 293 cells (HEK293). It contains AA His 109 - Thr 270 (Accession # O95760-1(C208S,C227S,C232S,C259S)).
Predicted N-terminus: His 109
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.2 kDa. The protein migrates as 25-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介绍
白细胞介素33（IL33）又称C9orf26、DKFZp586H0523、DVS27、NF-HEV、NFEHEV、RP11-575C20.2，是隶属于IL-1超家族的细胞因子。它能诱导辅助T细胞、肥大细胞、嗜酸性粒细胞和嗜碱性粒细胞产生2型细胞因子。IL-33通过与其受体ST2（即IL1RL1）及IL-1受体辅助蛋白（IL1RAP）相互作用，激活NF-κB和MAP激酶信号通路中的细胞内分子，从而促使极化Th2细胞产生2型细胞因子（如IL-5和IL-13）。在体内环境中，IL-33可诱导IL-4、IL-5和IL-13的表达，并导致黏膜器官发生严重病理改变。
关键字： IL-33;IL-33蛋白;IL-33重组蛋白;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group（股票代码：301080）是成立于2010年的跨国生物科技公司，是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球，横跨亚洲、北美洲、欧洲，在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家，与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。