Lactulose synthesis
- Product Name:Lactulose
- CAS Number:4618-18-2
- Molecular formula:C12H22O11
- Molecular Weight:342.3
63-42-3
569 suppliers
$36.00/100g
4618-18-2
432 suppliers
$28.00/1g
Yield:-
Reaction Conditions:
with β-galactosidase from kluyveromyces lactis;glucose isomerase from streptomyces rubiginosus in aq. phosphate buffer; pH=7.5 at 53.5; for 3 h;Catalytic behavior;Enzymatic reaction;Temperature;Concentration;
Steps:
Lactulose synthesis
The following reaction conditions were used for lactulose synthesis with the immobilized enzymes: lactose (14%, 16%, 18%, and 20%, w/v), temperature (47, 50, 53.5, 57, and 60 °C), ionic strength (50, 100, 150, 200, 250, and 300 mM) of sodium phosphate buffer at pH 7.5, ratio (1:1, 1:2, 1:3, 1:4, 1:5, and 1:6) of immobilized enzymes, agitation speed of 150 rpm, and a reaction time of 3 h. The lactose conversion rate (defined as ([S0]-[Ss])/[S0] × 100 %) and specific productivity (defined as [P]/([E] × t), mg/U h) were calculated. To test the reusability of the immobilized enzymes, they were filtered and washed with 100 mM sodium phosphate buffer (pH 7.5) between batch reactions. The relative catalytic activity of the immobilized enzymes was calculated as the lactulose ratio of the reused batch to the first batch, and was expressed as a percentage. All experiments were conducted in duplicate, and data were represented as an error bar.
References:
Song, Yoon-Seok;Lee, Hee-Uk;Park, Chulhwan;Kim, Seung-Wook [Carbohydrate Research,2013,vol. 369,p. 1 - 5]
57-48-7
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$12.00/250G
63-42-3
569 suppliers
$36.00/100g
4618-18-2
432 suppliers
$28.00/1g
5965-66-2
111 suppliers
$37.40/50g
87-81-0
386 suppliers
$12.50/5g
50-99-7
1062 suppliers
$6.00/25g
59-23-4
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$6.00/25g
4618-18-2
432 suppliers
$28.00/1g