4-Nitrofluorescein
- CAS No.
- 3326-35-0
- Chemical Name:
- 4-Nitrofluorescein
- Synonyms
- 4-NITROFLUORESCEIN;4-NITROFLUOROESCEIN;5-Nitrofluorescein (isomer I);5-Nitrofluorescein(isomerI)>3',6'-dihydroxy-6-nitrospiro[2-benzofuran-3,9'-xanthene]-1-one;3',6'-dihydroxy-6-nitro-spiro[2-benzofuran-3,9'-xanthene]-1-one;3',6'-dihydroxy-6-nitro-spiro[isobenzofuran-3,9'-xanthene]-1-one;6,11-dioxo-12-naphtho[2,3-b]indolizinecarboxylic acid ethyl ester;3',6'-Dihydroxy-5-nitro-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one;3',6'-Dihydroxy-5-nitrospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
- CBNumber:
- CB8768159
- Molecular Formula:
- C20H11NO7
- Molecular Weight:
- 377.3
- MDL Number:
- MFCD00135108
- MOL File:
- 3326-35-0.mol
- MSDS File:
- SDS
- TDS File:
- TDS
| Melting point | >300°C |
|---|---|
| Boiling point | 692.0±55.0 °C(Predicted) |
| Density | 1.72±0.1 g/cm3(Predicted) |
| storage temp. | 2-8°C |
| solubility | almost transparency in NaOH100g/L |
| form | powder to crystal |
| pka | 9.29±0.20(Predicted) |
| color | Light yellow to Brown |
| Stability | Light and Moisture Sensitive |
| InChI | InChI=1S/C20H11NO7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)20(15)14-4-1-10(21(25)26)7-13(14)19(24)28-20/h1-9,22-23H |
| InChIKey | UACQOMKZFGNRBL-UHFFFAOYSA-N |
| SMILES | C12(C3=C(C=C(O)C=C3)OC3=C1C=CC(O)=C3)C1=C(C=C([N+]([O-])=O)C=C1)C(=O)O2 |
| EPA Substance Registry System | Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-nitro- (3326-35-0) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|---|---|
| Signal word | Warning |
| Hazard statements | H302-H315-H319-H335 |
| Precautionary statements | P280-P305+P351+P338 |
| TSCA | TSCA listed |
| HS Code | 2932.99.7000 |
4-Nitrofluorescein price More Price(31)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| TCI Chemical | N0199 | 5-Nitrofluorescein (isomer I) >98.0%(T) | 3326-35-0 | 1g | $194 | 2026-04-30 | Buy |
| TCI Chemical | N0199 | 5-Nitrofluorescein (isomer I) >98.0%(T) | 3326-35-0 | 5g | $679 | 2026-04-30 | Buy |
| Usbiological | 285230 | Nitrofluorescein, isomer 1 | 3326-35-0 | 500mg | $410 | 2026-06-03 | Buy |
| Usbiological | 285230 | Nitrofluorescein, isomer 1 | 3326-35-0 | 1g | $602 | 2026-06-03 | Buy |
| Usbiological | 285230 | Nitrofluorescein, isomer 1 | 3326-35-0 | 2g | $904 | 2026-06-03 | Buy |
4-Nitrofluorescein Chemical Properties, Uses, Production
Chemical Properties
Orange Cyrstalline Solid
Uses
Fluorescent labeling reagent for proteins. 4-Nitrofluorescein is used in the fluorescent antibody technique for rapid identification of pathogens.
Synthesis
4-Nitrofluorescein was prepared as follows: 8.6 g (18.7 mmol) of ethyl 4-nitrofluorescein triacetate was dissolved in 87 mL of 10% sodium hydroxide solution and 432 mL of methanol and gently heated. The precipitate was filtered and dissolved in 500 mL of water. The solution was acidified with hydrochloric acid and allowed to stand overnight in the dark. The crystals were filtered out and washed with water. 6.9 g (18.2 mmol), 97% red crystals of 4-nitrofluorescein were obtained.C20H11NO7; molecular weight: 377.30; 1HNMR (300 MHz, DMSO-d6)δ 10.20 (brs, 2H, OH), 8.65 (s, 1H, nsi-4H), 8.57 (d, J=8.4. 1H, nsi-6H), 7.57 (d, J=8.3, 1H, nsi-7H), 6.70-6.64 (m, 4H, xa-4, 5, 2, 7H), 6.56 (d, J=8.7, 2H, xa-1, 8H). esims: 376.1 [M-H+] (100).
4-Nitrofluorescein Preparation Products And Raw materials
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| J & K SCIENTIFIC LTD. | 18210857532 18210857532 | jkinfo@jkchemical.com | China | 96815 | 76 |
| Meryer (Shanghai) Chemical Technology Co., Ltd. | 4006356688 18621169109 | market03@meryer.com | China | 40202 | 62 |
| TCI (Shanghai) Development Co., Ltd. | 021-67121386 | Sales-CN@TCIchemicals.com | China | 24512 | 81 |
| Energy Chemical | 400-0056266 | sales8178@energy-chemical.com | China | 44850 | 61 |
| Nanjing Habo Medical Technology Co., Ltd. | 13376080562 13376090521 | sales@habotech.com | China | 726 | 60 |
| Chemsky（shanghai）International Co.,Ltd. | 021-50135380 | shchemsky@sina.com | China | 32273 | 50 |
| Shandong Xiya Chemical Co., Ltd | 13355009207 13355009207 | 3007715519@qq.com | China | 18722 | 57 |
| Nanjing Norris-Pharm Technology Co., Ltd | 13901585132 | sales@norris-pharm.com | China | 8869 | 55 |
| Shanghai Jian Chao Chemical Technology Co., Ltd. | 150-2103-5486 18017383231 | 983544897@qq.com | China | 9336 | 55 |
| TOKYO CHEMICAL INDUSTRY CO., LTD. | 03-36680489 | Sales-JP@TCIchemicals.com | Japan | 28364 | 80 |
View Latest Price from 4-Nitrofluorescein manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2024-07-11 | 4-Nitrofluorescein
3326-35-0
|
$1.00 | 1KG | 99% | 20T | Shaanxi Dideu Medichem Co. Ltd |
-
- 4-Nitrofluorescein
3326-35-0
- $1.00
- 99%
- Shaanxi Dideu Medichem Co. Ltd
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