DICUMAROL
- CAS No.
- 66-76-2
- Chemical Name:
- DICUMAROL
- Synonyms
- DICOUMAROL;DICOUMARIN;nc034;Melitoxin;Cuma;Cumid;Acadyl;Acavyl;Dicuman;Dicumol
- CBNumber:
- CB9224757
- Molecular Formula:
- C19H12O6
- Molecular Weight:
- 336.3
- MDL Number:
- MFCD00006857
- MOL File:
- 66-76-2.mol
- MSDS File:
- SDS
- TDS File:
- TDS
| Product description | Number | Pack Size | Price |
| 3,3′-Methylene-bis(4-hydroxycoumarin) | M1390 | 5g | $62.6 |
| Dicoumarol | 287897 | 500mg | $94 |
| Dicoumarol | M0216 | 1G | $34 |
| Dicoumarol | M0216 | 25G | $315 |
| Dicoumarol ≥98% | 20764 | 1g | $20 |
| More product size | |||
| Melting point | 290-292 °C(lit.) |
|---|---|
| Boiling point | 392.79°C (rough estimate) |
| Density | 1.2864 (rough estimate) |
| refractive index | 1.4450 (estimate) |
| storage temp. | Sealed in dry,Room Temperature |
| solubility |
DMSO:3.06(Max Conc. mg/mL);9.1(Max Conc. mM) DMSO:PBS (pH 7.2) (1:1):0.5(Max Conc. mg/mL);1.49(Max Conc. mM) DMF:1.25(Max Conc. mg/mL);3.72(Max Conc. mM) Water:50.0(Max Conc. mg/mL);148.68(Max Conc. mM) |
| pka | 4.20±1.00(Predicted) |
| form | Fine Crystalline Powder |
| color | White |
| biological source | synthetic (organic) |
| Water Solubility | Soluble in aqueous alkaline solutions, organic bases, 0.1 N NaOH (15 mg/ml), Pyridine (50 mg/ml), chloroform (slightly soluble), and benzene (slightly soluble). Insoluble in water, and alcohols. |
| Merck | 14,3090 |
| Henry's Law Constant | 7.0×107 mol/(m3Pa) at 25℃, HSDB (2015) |
| InChI | 1S/C19H12O6/c20-16-10-5-1-3-7-14(10)24-18(22)12(16)9-13-17(21)11-6-2-4-8-15(11)25-19(13)23/h1-8,20-21H,9H2 |
| InChIKey | DOBMPNYZJYQDGZ-UHFFFAOYSA-N |
| SMILES | [o]1c2c(c(c([c]1=O)Cc3[c]([o]c4c(c3O)cccc4)=O)O)cccc2 |
| EWG's Food Scores | 3-6 |
| FDA UNII | 7QID3E7BG7 |
| ATC code | B01AA01 |
| Proposition 65 List | Dicumarol |
| EPA Substance Registry System | 2H-1-Benzopyran-2-one, 3,3'-methylenebis[4-hydroxy- (66-76-2) |
| UNSPSC Code | 12352200 |
| NACRES | NA.77 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |    GHS06,GHS08,GHS09 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Danger | |||||||||
| Hazard statements | H301-H372-H411 | |||||||||
| Precautionary statements | P260-P264-P270-P273-P301+P310-P314 | |||||||||
| Hazard Codes | T,N | |||||||||
| Risk Statements | 22-48/25-51/53 | |||||||||
| Safety Statements | 37-45-61 | |||||||||
| RIDADR | UN 2811 6.1/PG 3 | |||||||||
| WGK Germany | 3 | |||||||||
| RTECS | GN7875000 | |||||||||
| TSCA | TSCA listed | |||||||||
| HazardClass | 6.1(b) | |||||||||
| PackingGroup | III | |||||||||
| HS Code | 29322985 | |||||||||
| Storage Class | 6.1C - Combustible acute toxic Cat.3 toxic compounds or compounds which causing chronic effects |
|||||||||
| Hazard Classifications | Acute Tox. 3 Oral Aquatic Chronic 2 STOT RE 1 Oral |
|||||||||
| Hazardous Substances Data | 66-76-2(Hazardous Substances Data) | |||||||||
| Toxicity | LD50 orally in rats: 541.6 mg/kg (Rose) | |||||||||
| NFPA 704 |
|
DICUMAROL price More Price(22)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | M1390 | 3,3′-Methylene-bis(4-hydroxycoumarin) | 66-76-2 | 5g | $62.6 | 2026-03-19 | Buy |
| Sigma-Aldrich | 287897 | Dicoumarol | 66-76-2 | 500mg | $94 | 2026-03-19 | Buy |
| TCI Chemical | M0216 | Dicoumarol | 66-76-2 | 1G | $34 | 2026-03-19 | Buy |
| TCI Chemical | M0216 | Dicoumarol | 66-76-2 | 25G | $315 | 2026-03-19 | Buy |
| Cayman Chemical | 20764 | Dicoumarol ≥98% | 66-76-2 | 1g | $20 | 2024-03-01 | Buy |
DICUMAROL Chemical Properties,Uses,Production
Description
The plants containing dicoumarol mainly include red carnation grass (Trifolium pratense L., hongchezhoucao), rotten alfalfa (Medicago sativa L., zimuxu), rotten white vanilla rhinoceros (Melilotus albus Desr., baixiangcaomuxi), and other plants in Leguminosae.
Description
Dicoumarol is a competitive inhibitor of NADH:quinone oxidoreductase (NQO1) with IC50 values of 2.6 and 404 nM in the absence and presence of 2 μM BSA, respectively. It has antiproliferative activity against MIA PaCa-2 pancreas and HCT116 colon carcinoma cells (IC50s = 52 and 19 μM, respectively, after a 96 hour incubation). Dicoumarol inhibits stress-activated protein kinase (SAPK) in HEK293 cells (IC50 = 19-33 μM) at a point upstream of MEKK1 and downstream of TNF receptor-associated factor 2 (TRAF2), and it inhibits TNF-α and LPS-induced NF-κB activation in HeLa cells. It also has antiproliferative activity against FL5.12 lymphocytic and MCF-7 breast carcinoma cells (100 μM) by suppressing JNK activation.
Chemical Properties
white fine crystalline powder
Physical properties
Appearance: white or milky white crystalline powder, slightly fragran; Solubility: not dissolved in water, ethanol, and ether, slightly dissolved in chloroform, dissolved in alkali solution; Melting point: 287–293°C.It can be bluer or with purple fluorescence in the ultraviolet light.
History
In 1940, Karl Paul Link, a fertile scientist from the University of Wisconsin in the
United States, first isolated the anticoagulant substance from the moldy alfalfa
(Melilotus) and determined its structure. It is a kind of dicoumaroloid substance,
combined by two molecules of coumarin substances. Since this material was found
in the first few years, it has been used as a rodenticide .
In 1979, Conrad etal. reacted p-nitrobenzene ketone with 4-hydroxycoumarin to
obtain vinegar coumarin, which is basically the same as warfarin in anticoagulant,
but its metabolites also have anticoagulant effect, so the duration of anticoagulation
is longer than warfarin.
Uses
Dicumarol is a natural chemical used as an anticoagulant agents that functions as a vitamin K antagonist and is also a derivative of Coumarin.
Uses
This drug is used for preventing and treating thrombosis, thrombophlebitis, thromboemolium, and for preventing thrombo-formation in post-operational periods.
Indications
Intravascular thromboembolic diseases include postoperative or postoperative thrombotic phlebitis, pulmonary embolism, myocardial infarction, and atrial fibrillation caused by embolism.
Definition
ChEBI: A hydroxycoumarin that is methane in which two hydrogens have each been substituted by a 4-hydroxycoumarin-3-yl group.
General Description
Dicumarol, 3,3＇-methylenebis[4-hydroxycoumarin],is a white or creamy white crystalline powderwith a faint, pleasant odor and a slightly bitter taste. It ispractically insoluble in water or alcohol, slightly soluble inchloroform, and dissolved readily by solutions of fixed alkalies.The effects after administration require 12 to 72 hours todevelop and persist for 24 to 96 hours after discontinuance.
Biochem/physiol Actions
Prototype of the 4-hydroxycoumarin class of anticoagulants, which act as vitamin K antagonists, preventing formation of prothrombin. There are many reports that dicumarol also inhibits NADPH:quinone oxidoreductase (NQO(1)). In one, it inhibited NQO(1) in a pancreatic cancer cell line, causing increased formation of superoxide and inhibiting cell growth.
Pharmacology
Dicoumarin is an oral anticoagulant drug and is invalid invitro . Dicoumarin is a coumarin derivative, and its common mechanism is to inhibit synthesis of the coagulation factor in the liver. The structure of dicoumarin is similar to that of vitamin K and is an antagonist or a competitive inhibitor of vitamin K.It binds to the vitamin K epoxide reductase in the liver, inhibits the conversion of vitamin K from epoxide to hydroquinone, and inhibits the recycling of vitamin K, resulting in that the glutamate side chain of vitamin K-dependent coagulation factors II, VII, IX, and X cannot be carboxylated by γ-carboxy glutamate groups, affecting the binding of coagulation factor with calcium ion, and thereby inhibiting coagulation, reducing platelet adhesion, and prolonging thrombosis time . Dicoumarol drugs have no direct confrontation with synthesized prothrombin and coagulation factor, so it is ineffective invitro. After withdrawal of dicoumarin, prothrombin and coagulation factors II, IV, IX, and X gradually restore to a certain level, and hence the anticoagulant effect disappear, so its efficacy can be maintained for a long time .
Pharmacokinetics
Dicoumarol is not completely absorbed in the gastrointestinal tract, often is associated with gastrointestinal discomfort, and is very rarely used clinically. Today, the only coumarin used in the United States is warfarin, but phenprocoumon and acenocomumarol are used in Europe.
Clinical Use
Dicumarol is used alone or as an adjunct to heparin in theprophylaxis and treatment of intravascular clotting. It is usedin postoperative thrombophlebitis, pulmonary embolus, acuteembolic and thrombotic occlusion of peripheral arteries, andrecurrent idiopathic thrombophlebitis. It has no effect on analready-formed embolus but may prevent further intravascularclotting. Because the outcome of acute coronary thrombosisdepends largely on extension of the clot and formation ofmural thrombi in the heart chambers, with subsequent embolization,dicumarol has been used in this condition. It hasalso been administered to arrest impending gangrene afterfrostbite. The dose, after determination of the prothrombinclotting time, is 25 to 200 mg, depending on the size and thecondition of the patient. The drug is given orally in the formof capsules or tablets. On the second day and thereafter, itmay be given in amounts sufficient to maintain the prothrombinclotting time at about 30 seconds. If hemorrhages shouldoccur, a dosage of 50 to 100 mg of menadione sodium bisulfiteis injected, supplemented by a blood transfusion.
Side effects
The most serious adverse reaction of warfarin is bleeding, which can be against by vitamin K, and if necessary, fresh plasma or whole blood can be injected into the body to confront bleeding .
Synthesis
Dicoumarol, 3,3-methylene-bis(4-hydroxycoumarin) (24.1.8), is synthesized from 4-hydroxycoumarine (24.1.7), which is in turn synthesized from salicylic acid methyl ester by cyclization to a chromone derivative using sodium or sodium methoxide; or from o-oxyacetophenone by reacting it with diethylcarbonate in the presence of sodium ethoxide. Condensation of the resulting 4-hydroxycoumarin with formaldehyde as a phenol component gives dicoumarol.
References
[1] KAREN A. NOLAN. Synthesis and Biological Evaluation of Coumarin-Based Inhibitors of NAD(P)H: Quinone Oxidoreductase-1 (NQO1)[J]. Journal of Medicinal Chemistry, 2009, 52 22: 7142-7156. DOI: 10.1021/jm9011609
[2] J V CROSS. Quinone reductase inhibitors block SAPK/JNK and NFkappaB pathways and potentiate apoptosis.[J]. The Journal of Biological Chemistry, 1999, 274 44: 31150-31154. DOI: 10.1074/jbc.274.44.31150
[3] D. KRAUSE. Transient Activation of Jun N-terminal Kinases and Protection from Apoptosis by the Insulin-like Growth Factor I Receptor Can Be Suppressed by Dicumarol*[J]. The Journal of Biological Chemistry, 2001, 27 1: 19244-19252. DOI: 10.1074/jbc.m008186200
DICUMAROL Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| career henan chemical co | +86-0371-86658258 +8613203830695 | sales@coreychem.com | China | 29819 | 58 |
| Chengdu GLP biotechnology Co Ltd | 028-87075086 13350802083 | scglp@glp-china.com | CHINA | 1824 | 58 |
| Shenzhen Nexconn Pharmatechs Ltd | +86-755-89396905 +86-15013857715 | admin@nexconn.com | China | 10404 | 58 |
| Chengdu Biopurify Phytochemicals Ltd. | +86-28-82633860; +8618080483897 | sales@biopurify.com | China | 3751 | 58 |
| Shaanxi Dideu Medichem Co. Ltd | +86-29-87569262 +86-15003564040 | 1056@dideu.com | China | 3982 | 58 |
| TargetMol Chemicals Inc. | +1-781-999-5354; +17819995354 | marketing@targetmol.com | United States | 32467 | 58 |
| Hefei TNJ Chemical Industry Co.,Ltd. | +86-0551-65418671 +8618949823763 | sales@tnjchem.com | China | 34563 | 58 |
| changzhou huayang technology co., ltd | +8615250961469 | 2571773637@qq.com | China | 9653 | 58 |
| InvivoChem | +1-708-310-1919 +1-13798911105 | sales@invivochem.cn | United States | 6391 | 58 |
| HANGZHOU LEAP CHEM CO., LTD. | +86-571-87711850 | market18@leapchem.com | China | 24597 | 58 |
View Lastest Price from DICUMAROL manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2026-04-22 | Dicoumarol
66-76-2
|
US $41.00 / mg | 98.11% | 10g | TargetMol Chemicals Inc. | ||
 |
2026-04-22 | Dicoumarol
66-76-2
|
US $41.00 / mg | 98.11% | 10g | TargetMol Chemicals Inc. | ||
 |
2025-06-03 | Dicoumarol
66-76-2
|
US $41.00 / mg | 100% | 10g | TargetMol Chemicals Inc. |
-
- Dicoumarol
66-76-2
- US $41.00 / mg
- 98.11%
- TargetMol Chemicals Inc.
-
- Dicoumarol
66-76-2
- US $41.00 / mg
- 98.11%
- TargetMol Chemicals Inc.
-
- Dicoumarol
66-76-2
- US $41.00 / mg
- 100%
- TargetMol Chemicals Inc.
