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| | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Basic information |
| Product Name: | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt | | Synonyms: | 5-BROMO-4-CHLORO-3-INDOYL PHOSPHATE, P-TOLUIDINE SALT;5-BROMO-4-CHLORO-3-INDOXYL PHOSPHATE, P-TOLUIDINE SALT;5-BROMO-4-CHLORO-1H-INDOL-3-YL PHOSPHATE P-TOLUIDINE;5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE;5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE SALT;5-BROMO-4-CHLORO-3-INDOLYL-PHOSPHATE 4-TOLUIDINE SALT;5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE, COMPOUNDED WITH 4-METHYLBENZENAMINE;5-BROMO-4-CHLORO-3'-INDOLYPHOSPHATE P-TOLUIDINE SALT | | CAS: | 6578-06-9 | | MF: | C8H6BrClNO4P.C7H9N | | MW: | 433.63 | | EINECS: | 229-506-1 | | Product Categories: | substrate;Indoles;Simple Indoles | | Mol File: | 6578-06-9.mol |  |
| | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Chemical Properties |
| Melting point | 194-195℃ | | storage temp. | -20°C | | solubility | DMF: 20 mg/mL | | form | tablets | | color | White to Orange to Green | | InChI | InChI=1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3 | | InChIKey | QEIFSLUFHRCVQL-UHFFFAOYSA-N | | SMILES | C12C(Cl)=C(C=CC=1NC=C2OP(=O)(O)O)Br.C1(C)C=CC(N)=CC=1 | | CAS DataBase Reference | 6578-06-9(CAS DataBase Reference) | | EPA Substance Registry System | 1H-Indol-3-ol, 5-bromo-4-chloro-, dihydrogen phosphate (ester), compd. with 4-methylbenzenamine (1:1) (6578-06-9) |
| Hazard Codes | Xi | | Risk Statements | 36/37/38-40 | | Safety Statements | 26 | | RIDADR | 2811 | | WGK Germany | 3 | | F | 3-8-10 | | TSCA | TSCA listed | | HazardClass | 6.1 | | PackingGroup | III | | HS Code | 29339190 | | Storage Class | 11 - Combustible Solids |
| | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Usage And Synthesis |
| Chemical Properties | White to light brown powder | | Uses | For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid. | | General Description | BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected. | | Biochem/physiol Actions | 5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity. It can be used as a substrate to monitor secreted embryonic alkaline phosphataseactivity in solution. |
| | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Preparation Products And Raw materials |
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