|
|
| | 5-TAMRA cadaverine Basic information |
| Product Name: | 5-TAMRA cadaverine | | Synonyms: | TETRAMETHYLRHODAMINE-5-CARBOXAMIDE CADAVERINE;5-TAMRA cadaverine | | CAS: | | | MF: | C30H36N4O4 | | MW: | 516.63 | | EINECS: | | | Product Categories: | | | Mol File: | Mol File |  |
| | 5-TAMRA cadaverine Chemical Properties |
| solubility | DMSO, DMF, MeOH |
| | 5-TAMRA cadaverine Usage And Synthesis |
| Description | 5-TAMRA cadaverine can used to modify carboxylic acid group in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. It also can be reversibly coupled to aldehydes and ketones to form a Schiff base – which can be reduced to a generate stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3).Although the mixed isomers of 5(6)-TAMRA cadaverine is a preferred, routinely used orange-fluorescent dye for staining proteins, it is rearly used for labeling peptides and nucleotides. Purification of 5(6)-TAMRA labeled peptide and nucleotides might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes. | | Chemical Reactivity | Primary amines | | Abs/Em Maxima | 553/575 nm | | Extinction Coefficient | 91,000 cm-1M-1 | | Spectrally Similar Dyes | Alexa Fluor® 546, TAMRA, CF™ 543, MB™ 543 |
| | 5-TAMRA cadaverine Preparation Products And Raw materials |
|