Ac-Arg-Leu-Arg-AMC

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Ac-Arg-Leu-Arg-AMC Basic information
Product Name:Ac-Arg-Leu-Arg-AMC
Synonyms:Ac-Arg-Leu-Arg-AMC;Ac-Arg-Leu-Arg-AMC trifluoroacetate salt;N2-Acetyl-L-arginyl-L-leucyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-argininamide;L-Argininamide,N2-acetyl-L-arginyl-L-leucyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-;Ac-RLR-AMC
CAS:929903-87-7
MF:C30H46N10O6
MW:642.75
EINECS:
Product Categories:
Mol File:929903-87-7.mol
Ac-Arg-Leu-Arg-AMC Structure
Ac-Arg-Leu-Arg-AMC Chemical Properties
density 1.39±0.1 g/cm3(Predicted)
pka13.29±0.46(Predicted)
color Solid lyophilized powder
SequenceAc-Arg-Leu-Arg-AMC
Safety Information
MSDS Information
Ac-Arg-Leu-Arg-AMC Usage And Synthesis
UsesAc-RLR-AMC (Ac-Arg-Leu-Arg-AMC) is a fluorogenic substrate for the 26S proteasome (Ex/Em: 380/440-460 nm). AMC is released upon cleavage, and its fluorescence can be used to quantify the trypsin-like activity of 26S proteasomes[1].
Biological ActivityFluorogenic tri-peptide substrate for measuring the trypsin-like peptidase activity of the 20S proteasome. It is cleaved exclusively at the amide R-AMC bond to release fluorescent product. Shows low Km and high specific activity. Excitation: 380nm. Emission: 460nm. This substrate is useful for inhibitor screening and kinetic analysis.
storage+4°C
References[1] Chang TL. Inhibitory effect of flavonoids on 26S proteasome activity. J Agric Food Chem. 2009 Oct 28;57(20):9706-15. DOI:10.1021/jf9017492
Ac-Arg-Leu-Arg-AMC Preparation Products And Raw materials
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