Product Details
| Product Name: Bromophenol Blue Sodium Salt | CAS No.: 34725-61-6 |
| EC-No.: 252-170-2 | Min. Order: 1kg |
| Purity: PH: 3.0-4.6 | Supply Ability: 100KGS |
| Release date: 2026/01/15 |
Bromophenol Blue Sodium Salt is a synthetic dye and pH indicator widely used in laboratory settings. Here's a concise breakdown:
Chemical Classification:
Sodium salt of Bromophenol Blue (a triphenylmethane dye).
Chemical formula:C19H9Br4O5S⋅Na (sodium salt form).
CAS Number: 62625-28-9.
Key Properties:
pH Indicator:
Transition range: pH 3.0 (yellow) to 4.6 (blue-purple).
Used to monitor acidity in titrations or buffer systems.
Electrophoresis Tracking Dye:
Added to DNA/RNA/protein samples to visualize migration progress in agarose or polyacrylamide gels.
Migrates at ~300–500 bp (DNA) or ~3–4 kDa (proteins), depending on gel conditions.
Solubility:
Highly water-soluble (due to sodium salt form) compared to the free acid.
Applications:
Molecular Biology: Tracking dye in gel electrophoresis.
Analytical Chemistry: pH indicator in titrations or buffer prep.
Microscopy: Staining agent for biological specimens (less common).
Safety & Handling:
Hazards: May cause mild skin/eye irritation. Avoid inhalation.
Precautions: Use gloves, lab coat, and eye protection.
Disposal: Follow local regulations for chemical waste.
Key Notes:
Often mixed with other dyes (e.g., Xylene Cyanol) in loading buffers for electrophoresis.
Not suitable for neutral/basic pH ranges (use Bromothymol Blue or Phenolphthalein instead).
Let me know if you need further details!


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